The induction of oxidative stress and cellular death by the drinking water disinfection by-products, dichloroacetate and trichloroacetate in J774.A1 cells
The induction of oxidative stress and cellular death by the drinking water disinfection by-products, dichloroacetate and trichloroacetate in J774.A1 cells
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DOI：
10.1016/s1532-0456(03)00082-6
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发表时间：
2003-06-01
影响因子：
3.9
通讯作者：
Ray, S
中科院分区：
文献类型：
--
作者：
Hassoun, EA;Ray, S
采用J774A.1巨噬细胞系研究了饮用水消毒副产物二氯乙酸（DCA）和三氯乙酸（TCA）的体外毒性。将浓度范围为8-32 mM的DCA和TCA加入细胞培养物中，孵育24、36和60 h。测定了DCA和TCA对细胞活力、乳酸脱氢酶（LDH）释放和超氧阴离子（SA）产生以及细胞超氧化物歧化酶（SOD）活性的影响。DCA和TCA引起细胞死亡、LDH释放和SA产生的细胞时间和浓度依赖性增加。这些化合物还引起细胞SOD活性的调节，在较低浓度和/或较短孵育时间内观察到增加，而在较高浓度和/或较长孵育时间内观察到抑制。研究结果表明，DCA和TCA诱导巨噬细胞活化，并且活化与细胞毒性相关。此外，发现DCA和TCA对J774.A1细胞是等毒性的。(C)2003年爱思唯尔科学公司All rights reserved.
The in vitro toxicity of the drinking water disinfection by products dichloroacetate (DCA) and trichloroacetate (TCA) were studied using the J774A.1 macrophage cell line. DCA and TCA were added to cell cultures at concentrations ranging between 8-32 mM and incubated for 24, 36 and 60 h. DCA and TCA effects on cellular viability, lactate dehydrogenase (LDH) release and superoxide anion (SA) production by the cells, as well as superoxide dismutase (SOD) activities of the cells were determined. DCA and TCA caused time- and concentration-dependent increases in cellular death, in LDH release and production of SA by the cells. The compounds also caused modulations in SOD activities of the cells, with increases observed at the lower concentrations and/or shorter periods of incubations and suppression with the higher concentrations and/or longer periods of incubation. The results of the study indicate that DCA and TCA induce macrophage activation and that the activation is associated with cellular toxicity. Also, DCA and TCA are found to be equitoxic to J774.A1 cells. (C) 2003 Elsevier Science Inc. All rights reserved.