Common Markers and Small Molecule Inhibitors in Golgi Studies.
Common Markers and Small Molecule Inhibitors in Golgi Studies.
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DOI：
10.1007/978-1-0716-2639-9_27
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发表时间：
2023
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在本章中，我们提供了应用常用小分子在体外研究高尔基体结构和功能的详细指南。此外，我们还整理了一份简明、经过验证的内膜标记物列表，这些标记物通常用于下游测定，以通过显微镜和蛋白质印迹检查药物治疗后对高尔基体的后续影响。本章对于开始涉足细胞内运输和高尔基体生物学领域的研究人员将很有用。
In this chapter, we provide a detailed guide for the application of commonly used small molecules to study Golgi structure and function in vitro. Furthermore, we have curated a concise, validated list of endomembrane markers typically used in downstream assays to examine the consequent effect on the Golgi via microscopy and western blot after drug treatment. This chapter will be useful for researchers beginning their foray into the field of intracellular trafficking and Golgi biology.