Synergism of human amnion-derived multipotent progenitor (AMP) cells and a collagen scaffold in promoting brain wound recovery: Pre-clinical studies in an experimental model of penetrating ballistic-like brain injury
Synergism of human amnion-derived multipotent progenitor (AMP) cells and a collagen scaffold in promoting brain wound recovery: Pre-clinical studies in an experimental model of penetrating ballistic-like brain injury
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DOI：
10.1016/j.brainres.2010.10.028
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发表时间：
2011-01-12
期刊：
影响因子：
2.9
通讯作者：
Tortella, Frank C.
中科院分区：
文献类型：
--
作者：
Chen, Zhiyong;Lu, X. -C. May;Tortella, Frank C.
穿透性弹道脑损伤的组织病理学结果之一是形成永久性空洞。在先前使用穿透性弹道样脑损伤（PBBI）模型的研究中，移植的人羊膜来源的多能祖细胞（AMP）在直接注射到损伤道时未能存活，这表明细胞存活需要支持基质。在这项研究中，我们将AMP细胞置于胶原基支架中，注射到损伤核心，研究PBBI后的细胞存活和神经保护作用。AMP细胞悬浮在AMP细胞条件培养基（ACCS）或液化胶原基质中，在PBBI后立即沿穿透性损伤道注射。受伤大鼠只接受液化胶原基质。所有的动物都被允许存活两周。与我们之前的结果一致，悬浮在ACCS中的AMP细胞未能存活；同样，单独注射时，在损伤部位未发现胶原蛋白。同时注射时，AMP细胞和胶原蛋白均保存在损伤腔内。此外，AMP细胞/胶原处理在损伤腔内保留了一些明显的脑组织，并且在保存的脑组织中有可测量的内源性神经祖细胞和星形胶质细胞浸润。AMP细胞也被发现迁移到脑室下区和胼胝体。此外，AMP细胞/胶原蛋白处理显著减轻了pbbi诱导的胼胝体和同侧丘脑轴突变性，改善了旋转机械性能的运动障碍。总之，胶原基支架为AMP细胞的存活、迁移和神经保护提供了支持基质。Elsevier B.V.出版
One of the histopathological consequences of a penetrating ballistic brain injury is the formation of a permanent cavity. In a previous study using the penetrating ballistic-like brain injury (PBBI) model, engrafted human amnion-derived multipotent progenitor (AMP) cells failed to survive when injected directly in the injury tract, suggesting that the cell survival requires a supportive matrix. In this study, we seated AMP cells in a collagen-based scaffold, injected into the injury core, and investigated cell survival and neuroprotection following PBBI. AMP cells suspended in AMP cell conditioned medium (ACCS) or in a liquefied collagen matrix were injected immediately after a PBBI along the penetrating injury tract. Injured control rats received only liquefied collagen matrix. All animals were allowed to survive two weeks. Consistent with our previous results, AMP cells suspended in ACCS failed to survive; likewise, no collagen was identified at the injury site when injected alone. In contrast, both AMP cells and the collagen were preserved in the injury cavity when injected together. In addition, AMP cells/collagen treatment preserved some apparent brain tissue in the injury cavity, and there was measurable infiltration of endogenous neural progenitor cells and astrocytes into the preserved brain tissue. AMP cells were also found to, have migrated into the subventricular zone and the corpus callosum. Moreover, the AMP cell/collagen treatment significantly attenuated the PBBI-induced axonal degeneration in the corpus callosum and ipsilateral thalamus and improved motor impairment on rotarod performance. Overall, collagen-based scaffold provided a supportive matrix for AMP cell survival, migration, and neuroprotection. Published by Elsevier B.V.