Phenolic content and antioxidant properties of soybean (Glycine max (L.) Merr.) seeds
Phenolic content and antioxidant properties of soybean (Glycine max (L.) Merr.) seeds
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DOI：
10.3390/12030576
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发表时间：
2007-03-01
期刊：
影响因子：
4.6
通讯作者：
Miladinovic, Jegor
中科院分区：
文献类型：
--
作者：
Malencic, Djordje;Popovic, Milan;Miladinovic, Jegor
测定了20个大豆杂交种种子中酚类物质的含量及其抗氧化能力。用70%丙酮水溶液提取种子后，用比色法测定总酚、单宁和原花青素。并测定了总黄酮的含量。采用1，1-二苯基-2-苦基肼（DPPH）自由基清除活性测定法，对水溶性丙酮提取物的抗氧化活性进行了评价。塞尔维亚品种1511和中国品种LN 92 -7369的总酚含量最高，总抗氧化活性也最高。相反，酚类物质的基因型穷人也表现出低水平的DPPH自由基清除活性。结果表明，除蛋白质和油分含量外，酚类物质含量也是大豆种子的重要特征，可作为大豆抗氧化活性的潜在选择指标。
The contents and antioxidant ability of various classes of phenolic compounds present in the seeds of twenty soybean hybrids were evaluated. Total phenolics, tannins and proanthocyanidins were determined spectrophotometrically, after extraction of seeds with 70% aqueous acetone. In addition, the flavonoid contents were determined. The antioxidant activity of aqueous acetone extracts was evaluated by the 1,1-diphenyl-2picrylhydrazyl (DPPH) free radical scavenging activity assay. The highest contents of total phenolics were found in Serbian cultivar 1511 and Chinese cultivar LN92-7369, which also displayed the highest total antioxidant activity. Conversely, genotypes poor in phenolics also showed low levels of DPPH-radical scavenging activity. The results suggested that besides protein and oil contents, the phenolic contents should be also considered as an important characteristic feature of soybean seeds, and as a potential selection criterion for antioxidant activity in soybean.