GLP-1 is localized to the mitotic region of the C. elegans germ line.
GLP-1 is localized to the mitotic region of the C. elegans germ line.
复制标题
DOI：
--
复制
发表时间：
1994-10
期刊：
影响因子：
4.6
通讯作者：
S. Crittenden;E. Troemel;T. C. Evans;J. Kimble
中科院分区：
文献类型：
--
作者：
S. Crittenden;E. Troemel;T. C. Evans;J. Kimble
In C.在线虫中，生殖系有丝分裂依赖于体细胞远端细胞（DTC）的诱导和glp-1基因的活性。使用GLP-1蛋白的抗体，我们已经检查了GLP-1的蛋白质印迹和免疫细胞化学。GLP-1与有丝分裂种系细胞的膜紧密相关，支持其作为一种完整的膜蛋白的鉴定。此外，GLP-1定位于生殖系内的有丝分裂区域，与GLP-1作为远端细胞信号的膜受体的模型一致。出乎意料的是，GLP-1和有丝分裂区比DTC过程延伸得更远。我们提出了三种模型，DTC可能会影响GLP-1活性，从而确定有丝分裂区。GLP-1的空间限制似乎在两性动物的翻译水平上受到控制。我们认为，GLP-1的下调可能需要影响从有丝分裂到减数分裂的过渡。
In C. elegans, germline mitosis depends on induction by the somatic distal tip cell (DTC) and on activity of the glp-1 gene. Using antibodies to GLP-1 protein, we have examined GLP-1 on western blots and by immunocytochemistry. GLP-1 is tightly associated with membranes of mitotic germline cells, supporting its identification as an integral membrane protein. Furthermore, GLP-1 is localized within the germ line to the mitotic region, consistent with the model that GLP-1 acts as a membrane receptor for the distal tip cell signal. Unexpectedly, GLP-1 and the zone of mitosis extend further than the DTC processes. We present three models by which the DTC may influence GLP-1 activity and thereby determine the zone of mitosis. The spatial restriction of GLP-1 appears to be controlled at the translational level in hermaphrodites. We suggest that down-regulation of GLP-1 may be required to effect the transition from mitosis into meiosis.