A Multiplexed, Gradient-Based, Full-Hydrogel Microfluidic Platform for Rapid, High-Throughput Antimicrobial Susceptibility Testing
A Multiplexed, Gradient-Based, Full-Hydrogel Microfluidic Platform for Rapid, High-Throughput Antimicrobial Susceptibility Testing
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用于快速、高通量抗菌药物敏感性测试的多重、基于梯度的全水凝胶微流体平台
DOI：
10.1002/cplu.201600654
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发表时间：
2017
期刊：
影响因子：
3.4
通讯作者：
Ren Kangning
中科院分区：
文献类型：
--
作者：
Liu Zhengzhi;Sun Han;Ren Kangning
随着抗菌素耐药性继续在全球蔓延，它已成为现代医疗保健系统的直接威胁。随着新型抗生素的开发停滞，保持现有药物的有效性已成为当务之急。抗生素耐药性背后的主要驱动力之一是抗生素的误用和过度使用，这通常是由于未能方便、及时地获得病原体敏感性数据而导致的，而传统的抗生素敏感性测试很难满足这一需求。在此，报道了一种用于抗菌敏感性测试目的的水凝胶微流体平台，能够处理真实样品并在培养 2.5 小时内产生结果。通过使用通道相互交叉的多人设计，可以在同一设备上进行多个一维或二维实验。将水凝胶表面生长的细菌转移到载玻片上后，可以通过标准革兰氏染色轻松观察到。与基于软件的图像分析相结合，该系统可以单独或组合地产生有关细菌敏感性和药物作用的各种有用信息，例如细菌的最低抑菌浓度和形态变化。与传统的检测方法相比，该系统操作所需的劳动力、试剂和设备更少，速度和效率显着提高。
Antimicrobial resistance has become an immediate threat to modern healthcare systems as it continues to spread across the globe. As development of novel antibiotics stalls, preserving the effectiveness of existing agents has become a priority. One of the major driving forces behind antimicrobial resistance is the misuse and overuse of antibiotics, often a result of data on the susceptibility of pathogens not being obtained in a convenient and timely manner, a need that conventional antimicrobial susceptibility testing struggles to meet. Here, a hydrogel microfluidic platform is reported for antimicrobial susceptibility testing purposes, capable of handling real samples and yielding results within 2.5 h of culture. By using a multiplayer design with channels crossing overhead of each other, multiple experiments, either one‐ or two‐dimensional, can be staged on the same device. Bacteria grown on the surface of the hydrogel can be easily visualized with standard Gram staining after being transferred onto a glass slide. Coupled with software‐based image analysis, the system can yield a variety of useful information on bacterial susceptibility and the effects of drugs, such as minimum inhibitory concentration and morphological changes in bacteria, either individually or in combination. Compared to conventional testing methods, this system requires less labor, reagents, and equipment to operate, and has significantly higher speed and efficiency.