Substrate specificity of alcohol dehydrogenases.
Substrate specificity of alcohol dehydrogenases.
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醇脱氢酶的底物特异性。
DOI：
10.1007/978-1-4615-2904-0_41
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发表时间：
1993
影响因子：
--
通讯作者：
Kim,K
中科院分区：
文献类型：
--
作者：
Plapp,BV;Green,DW;Sun,HW;Park,DH;Kim,K
醇脱氢酶具有重要的生理功能。因此，确定这些酶的底物特异性非常重要。通常，选择多种底物进行调查，并通过稳态动力学方法识别最具反应性的底物。乙醇是肝脏和酵母酶的良好底物，并且无疑是最重要的生理底物之一。然而，这些酶具有广泛的、实际上是混杂的特异性，作用于初级、次级和环状底物，甚至类固醇和半缩醛。因此，我们应该问哪些化合物是“正常”或“天然”底物，哪些好的、重要的底物尚未被发现？其中一些底物可能与酒精中毒的发病机制有关。
Alcohol dehydrogenases have important physiological fonctions. Thus, it is important to determine the substrate specificities of these enzymes. Usually, a variety of substrates are chosen for a survey, and the most reactive substrates are identified by steady-state kinetic methods. Ethanol is a good substrate for the liver and yeast enzymes and is certainly one of the most important physiological substrates. However, these enzymes have broad, indeed promiscuous, specificities, acting on primary, secondary, and cyclic substrates, even steroids and hemiacetals. Thus, we should ask which compounds are the “normal” or “natural” substrates, and what good, important substrates have not been discovered? Some of these substrates may be involved in the pathogenesis of alcoholism.