Synthesis of atypical cyclic and acyclic hydroxy carotenoids in Escherichia coli transformants
Synthesis of atypical cyclic and acyclic hydroxy carotenoids in Escherichia coli transformants
复制标题
DOI：
10.1016/s0168-1656(97)00151-x
复制
发表时间：
1997-12-03
影响因子：
4.1
通讯作者：
Sandmann, G
中科院分区：
文献类型：
--
作者：
Albrecht, M;Takaichi, S;Sandmann, G
共八种不同的羟基类胡萝卜素的非胡萝卜素的细菌大肠杆菌的转化体中产生。它们包括无环的1-羟基神经孢烯、1-羟基番茄红素、1，1 ′-二羟基番茄红素和去甲基类胡萝卜素以及环状的3-羟基-β-玉米胡萝卜素、7，8-二氢玉米黄质、3或3 ′-7，8-二氢-β-胡萝卜素和1 ′-羟基-γ-胡萝卜素。这些不常见的类胡萝卜素中的大多数在天然来源中仅以痕量存在。对于上述所有类胡萝卜素的合成，E.用最多三种相容质粒的组合转化大肠杆菌，所述质粒含有来自噬夏欧文氏菌和两种红细菌属物种的几种胡萝卜素生成基因。概述了它们在导致单个类胡萝卜素的途径中的功能。最后，生长条件进行了优化，生产的羟基类胡萝卜素的量，这是适合他们的分离和纯化。(C)1997年Elsevier Science B.V.
A total of eight different hydroxy carotenoids were produced in transformants of the non-carotenogenic bacterium Escherichia co ii. They include the acyclic 1-hydroxyneurosporene, 1-hydroxylycopene, 1,1'-dihydroxylycopene and demethylspheroidene as well as the cyclic 3-hydroxy-beta-zeacarotene, 7,8-dihydrozeaxanthin, 3 or 3'-7,8-dihydro-beta-carotene and 1'-hydroxy-gamma-carotene. Most of these uncommon carotenoids are found only in trace amounts in natural sources. For the synthesis of all the carotenoids mentioned above, E. coli was transformed with a combination of up to three compatible plasmids, which contained several carotenogenic genes from Erwinia uredovora and two Rhodobacter species. Their function in the pathway leading to the individual carotenoids was outlined. Finally, growth conditions were optimized for production of the hydroxy carotenoids in amounts which are suitable for their isolation and purification. (C) 1997 Elsevier Science B.V.