Early recruitment of phagocytes contributes to the vascular inflammation of giant cell arteritis
Early recruitment of phagocytes contributes to the vascular inflammation of giant cell arteritis
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DOI：
10.1002/path.1660
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发表时间：
2004-11-01
影响因子：
7.3
通讯作者：
Roth, J
中科院分区：
文献类型：
--
作者：
Foell, D;Hernández-Rodríguez, J;Roth, J
巨细胞动脉炎的血管炎症通常被描述为一个涉及树突状细胞、T淋巴细胞和效应组织巨噬细胞的过程。关于早期招募的吞噬细胞的作用，如单核细胞和中性粒细胞，人们知之甚少。这些细胞表达和分泌促炎的S100蛋白，直接激活内皮细胞。本研究对36例巨细胞动脉炎患者的巨细胞炎性细胞特异性促炎蛋白S100A8/S100A9和S100A12的表达进行了研究。此外，对42名患者和35名健康对照的血清样本中这些蛋白质的浓度进行了分析。S100A8/S100A9复合体在病变动脉的外膜和中膜中含量丰富。除了中性粒细胞外，表达这些蛋白的细胞属于CD68阳性单核细胞的促炎亚型。相反，S100A12的表达仅限于外膜内血管周围的中性粒细胞。巨细胞动脉炎患者血清S100A8/S100A9和S100A12浓度均显著高于正常对照组。总之，新近招募的表达促炎S100蛋白的吞噬细胞参与了巨细胞动脉炎的血管炎症。它们可能在受影响血管的血管中发挥重要作用，这些血管代表着新招募的炎症细胞的进入部位。这些数据表明，外膜和中膜内的吞噬细胞通过释放促炎蛋白S100A8、S100A9和S100A12参与炎症过程。版权所有(C)2004年大不列颠和爱尔兰病理学会。作者：John Wiley Sons，Ltd.
Vascular inflammation in giant cell arteritis is generally described as a process involving dendritic cells, T-lymphocytes, and effector tissue macrophages. Less is known about the contribution of phagocytes that are recruited early, such as monocytes and neutrophils. These cells express and secrete pro-inflammatory S100 proteins which directly activate endothelial cells. In this study the expression of S100A8/S100A9 and S100A12, proinflammatory proteins specific for early recruited phagocytes, was studied in biopsies from 36 patients with giant cell arteritis. In addition, serum concentrations of these proteins were analysed in serum samples from 42 patients and 35 healthy controls. The S100A8/S100A9 complex was found to be abundant in the adventitia and media in affected arteries. Besides neutrophils, cells expressing these proteins belonged to a pro-inflammatory subtype of CD68-positive monocytes. In contrast, S100A12 expression was restricted to neutrophils that were found around the vasa vasorum within the adventitial layer. Both S100A8/S100A9 and S100A12 serum concentrations were significantly higher in patients with giant cell arteritis than in healthy controls. In conclusion, recently recruited phagocytes expressing pro-inflammatory S100 proteins take part in the vascular inflammation of giant cell arteritis. They may play important roles at the vasa vasorum of affected vessels, which represent sites of entry for recruited inflammatory cells. These data indicate that phagocytes within the adventitia and media contribute to the process of inflammation via release of the proinflammatory S100 proteins S100A8, S100A9, and S100A12. Copyright (C) 2004 Pathological Society of Great Britain and Ireland. Published by John Wiley Sons, Ltd.