Analysis of promoter mutations in the histidine transport operon of Salmonella typhimurium: use of hybrid M13 bacteriophages for cloning, transformation, and sequencing.
Analysis of promoter mutations in the histidine transport operon of Salmonella typhimurium: use of hybrid M13 bacteriophages for cloning, transformation, and sequencing.
复制标题
鼠伤寒沙门氏菌组氨酸转运操纵子启动子突变分析：使用杂交 M13 噬菌体进行克隆、转化和测序。
DOI：
10.1128/jb.159.3.1000-1005.1984
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发表时间：
1984
影响因子：
3.2
通讯作者：
Ames,GF
中科院分区：
文献类型：
--
作者：
Lee,GS;Ames,GF
分离引起组氨酸转运操纵子表达水平增加的突变，并进行遗传学表征。将5个独立分离的启动子上调突变转移到携带组氨酸转运操纵子的M13杂交噬菌体中，并测定其核苷酸序列。对于所有五个突变，变化与先前确定的启动子上游突变dhuA1相同：Pribnow框中的C到T变化使该区域与共有序列更具同源性。本发明提供了使鼠伤寒沙门氏菌能够有效支持M13噬菌体生长和易于将染色体突变转移到杂交噬菌体上的方法。
Mutations that cause an increased level of expression of the histidine transport operon were isolated and characterized genetically. Five independently isolated promoter-up mutations were transferred to an M13 hybrid phage that carries the histidine transport operon, and their nucleotide sequences were determined. For all five mutations, the change was the same as the one previously determined for promoter-up mutation dhuA1: a C-to-T change in the Pribnow box rendered this region more homologous to the consensus sequence. Methods for enabling Salmonella typhimurium to support growth of M13 phage effectively and for easy transfer of chromosomal mutations onto the hybrid phage are presented.