Comparative proteomic analysis of tobacco expressing cyanobacterial flavodoxin and its wild type under drought stress
Comparative proteomic analysis of tobacco expressing cyanobacterial flavodoxin and its wild type under drought stress
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DOI：
10.1016/j.jplph.2014.11.001
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发表时间：
2015-03-01
影响因子：
4.3
通讯作者：
Salekdeh, Ghasem Hosseini
中科院分区：
文献类型：
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作者：
Gharechahi, Javad;Hajirezaei, Mohammad-Reza;Salekdeh, Ghasem Hosseini
表达蓝细菌黄氧还蛋白（flld）的烟草植株对干旱、温度和紫外线等多种非生物胁迫的耐受性增强。Fld表达对胁迫条件的适应机制在很大程度上是未知的。在此，我们应用比较蛋白质组学分析来揭示表达fld的植物在干旱胁迫下蛋白质组谱的变化。利用高分辨率二维凝胶电泳，我们能够检测到930个蛋白点并比较它们的丰度。我们发现转基因和/或野生型植物在干旱条件下的52个位点变化高达1.5倍。利用MALDI-TOF/TOF和ESI-Q/TOF联合分析，鉴定出39个干旱响应蛋白（DRPs），其中野生型24个，转基因11个，两者各4个。已知大多数DRPs参与光合作用、碳水化合物和能量代谢、氨基酸和蛋白质的合成和加工以及氧化应激反应。在候选DRPs中，干旱胁迫下fld烟草的remurin、铁氧化还原蛋白- nadp还原酶、叶绿体锰稳定蛋白- ii、磷酸甘油酸变化酶和谷胱甘肽s -转移酶丰度降低，而s -甲酰基谷胱甘肽水解酶和谷胱甘肽生物合成蛋白丰度增加。在野生型植物中，干旱导致与碳水化合物代谢相关的蛋白质减少。这些结果表明，Fld表达所赋予的胁迫耐受性与碳水化合物和能量代谢以及氧化应激反应的控制机制密切相关。(C) 2014 Elsevier GmbH版权所有。
Tobacco plants expressing cyanobacterial flavodoxin (Fld) show enhanced tolerance to a wide range of abiotic stresses including drought, temperature and UV. The mechanisms of adaptation to stress conditions under Fld expression are largely unknown. Here, we applied comparative proteomic analysis to uncover the changes in the proteome profile of Fld-expressing plants in response to drought stress. Using high-resolution two-dimensional gel electrophoresis, we were able to detect 930 protein spots and compare their abundance. We found changes up to 1.5 fold for 52 spots under drought in transgenic and/or wild type plants. Using combined MALDI-TOF/TOF and ESI-Q/TOF analysis 39 (24 in wild type, 11 in transgenic, and 4 in both) drought-responsive proteins (DRPs) could be identified. The majority of DRPs are known to be involved in photosynthesis, carbohydrate and energy metabolism, amino acid and protein synthesis and processing, and oxidative stress responses. Among candidate DRPs, the abundance of remurin, ferredoxin-NADP reductase, chloroplast manganese stabilizing protein-II, phosphoglycerate mutase, and glutathione S-transferase decreased in drought stressed Fld-tobacco while S-formylglutathione hydrolase and pyridoxine biosynthesis protein abundance increased. In wild type plants, drought caused a reduction of proteins related to carbohydrate metabolism. These results suggest that the stress tolerance conferred by Fld expression is strongly related to control mechanisms regarding carbohydrate and energy metabolism as well as oxidative stress responses. (C) 2014 Elsevier GmbH. All rights reserved.