Increased production of interleukin 6 and interleukin 8 in scleroderma fibroblasts
Increased production of interleukin 6 and interleukin 8 in scleroderma fibroblasts
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DOI：
10.1016/s0923-1811(98)84143-1
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发表时间：
1998-02-01
影响因子：
3.9
通讯作者：
Tamaki, K
中科院分区：
文献类型：
--
作者：
Kadono, T;Kikuchi, K;Tamaki, K
Objective.为了确定额外的异常特征相关的细胞因子在成纤维细胞来源于系统性硬化症（SSc），我们研究了白细胞介素6（IL-6）和IL-8的生产和它们的mRNA水平在硬皮病成纤维细胞和正常skin.Methods，培养成纤维细胞从患者SSc和健康对照进行了检查。采用特异性ELISA法检测IL-6和IL-8的产生，逆转录聚合酶链反应（RT-PCR）检测IL-6和IL-8 mRNA的表达。与对照组相比，硬皮病成纤维细胞中IL-6和IL-S的基础产生显著增加。当细胞被IL-1 β刺激时，（50 pg/ml）或肿瘤坏死因子-α（TNF-α：10 ng/ml）时，两种细胞株的IL-6和IL-8的产生均显著增加，但两种细胞株之间的IL-6和IL-8的产生无显著差异。IL-6和IL-8的产生均显着增加，即使在3次传代后，产量仍保持升高。RT-PCR结果显示，在无细胞因子刺激的情况下，硬皮病成纤维细胞表达IL-6和IL-8 mRNA，而正常皮肤成纤维细胞表达IL-6和IL-8 mRNA。硬皮病成纤维细胞产生IL-6和IL-8的增加表明这些细胞可能在体内受到某些细胞因子的刺激。
Objective. To determine additional abnormal characteristics related to cytokines in fibroblasts derived from systemic sclerosis (SSc), we examined the production of interleukin 6 (IL-6) and IL-8 and their mRNA levels both in scleroderma fibroblasts and in those from normal skin.Methods, Cultured fibroblasts from patients with SSc and healthy controls were examined. Production of IL-6 and IL-8 was assessed by specific ELISA, and the levels of IL-6 and IL-8 mRNA were determined by reverse transcriptase polymerase chain reaction (RT-PCR),Results. Basal production of both IL-6 and IL-S was significantly increased in scleroderma fibroblasts compared with controls. When cells were stimulated with either IL-1 beta (50 pg/ml) or tumor necrosis factor-alpha (TNF-alpha: 10 ng/ml), the production of IL-6 and IL-8 was predominantly increased in both cell strains and there was no significant difference in the production of IL-6 and IL-8 between them, When normal fibroblasts were stimulated with IL-IB for 48 h and subcultured, both IL-6 and IL-8 production were significantly increased, and production remained elevated even after 3 passages. RT-PCR revealed that IL-6 and IL-8 mRNA were detected in scleroderma fibroblasts but not in normal skin fibroblasts without cytokine stimulation, When stimulated with IL-1 beta, both cell strains expressed IL-6 and IL-8 mRNA to almost the same extent,Conclusion. Increased production of IL-6 and IL-8 by scleroderma fibroblasts suggests that these cells may have been stimulated by certain cytokines in vivo.