4-BASE CODONS ACCA, ACCU AND ACCC ARE RECOGNIZED BY FRAMESHIFT SUPPRESSOR SUFJ
4-BASE CODONS ACCA, ACCU AND ACCC ARE RECOGNIZED BY FRAMESHIFT SUPPRESSOR SUFJ
复制标题
DOI：
10.1016/0092-8674(81)90067-2
复制
发表时间：
1981-01-01
期刊：
影响因子：
64.5
通讯作者：
ROTH, JR
中科院分区：
文献类型：
--
作者：
BOSSI, L;ROTH, JR
鼠伤寒沙门氏菌sufJ的移码抑制子作用于纠正一组在突变位点具有不同序列的+1移码突变。该抑制子通过读取位于每个抑制性突变位点附近而不是位置的4碱基密码子起作用。因此，抑制需要在抑制tRNA的作用位点和移码突变位点之间进行短段mRNA的异相翻译。sufJ读取的位点是通过在先前不受抑制的移码突变附近突变创建一系列这样的位点来确定的。每个新生成的位点都是通过碱基置换形成的。通过DNA测序分析了四个独立产生的位点。在每个位点产生四联体密码子ACCX（其中X为A、U或C）。因此，sufJ能够读取4个碱基的密码子，其中3个碱基中的任何一个都可以在第4个位置上接受。这是第一个在密码子的前3个位置不读取3个重复碱基的移码抑制子。
The frameshift suppressor of Salmonella typhimurium sufJ acts to correct a set of +1 frameshift mutations having very different sequences at their mutant sites. This suppressor acts by reading a 4-base codon located near, but not at, the site of each suppressible mutation. Suppression thus necessitates out-of-phase translation of the short stretch of mRNA between the site of action of the suppressor tRNA and the site of the frameshift mutation. The site read by sufJ was identified by mutationally creating a series of such sites in the neighborhood of a previously nonsuppressible frameshift mutation. Each of the newly generated sites was formed by base substitution. Four independently generated sites were analyzed by DNA sequencing. At each site the quadruplet codon ACCX was generated (where X is A, U or C). Thus sufJ is able to read a 4-base codon in which any of 3 bases is acceptable in the 4th position. This is the 1st frameshift suppressor that does not read a run of 3 repeated bases in the first 3 positions of its codon.