Use of 16S ribosomal DNA for delineation of marine bacterioplankton species
Use of 16S ribosomal DNA for delineation of marine bacterioplankton species
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DOI：
10.1128/aem.68.7.3628-3633.2002
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发表时间：
2002-07-01
影响因子：
4.4
通讯作者：
Zweifel, UL
中科院分区：
文献类型：
--
作者：
Hagström, Å;Pommier, T;Zweifel, UL
重新分析了GenBank中所有的海洋细菌素衍生的16 S核糖体DNA序列，以确定海洋表面沃茨中细菌的物种数量。这些序列自1990年以来一直输入数据库。1999年，新增加的数量达到高峰，随后趋于平稳，这表明海洋微生物物种丰富度的大部分已被取样。当以97%相似性为界值对GenBank序列进行去复制时，发现了1,117个独特的核糖体型，其中609个来自未培养的环境克隆，508个来自培养的细菌。我们的结论是，浮游细菌的表观物种丰富度相对较低。
All of the marine bacterioplankton-derived 16S ribosomal DNA sequences previously deposited in GenBank were reanalyzed to determine the number of bacterial species in the oceanic surface waters. These sequences have been entered into the database since 1990. The rate of new additions reached a peak in 1999 and subsequently leveled off, suggesting that much of the marine microbial species richness has been sampled. When the GenBank sequences were dereplicated by using 97% similarity as a cutoff, 1,117 unique ribotypes were found. Of the unique sequences, 609 came from uncultured environmental clones and 508 came from cultured bacteria. We conclude that the apparent bacterioplankton species richness is relatively low.