The effects of Buyang Huanwu Decoction on hemorheological disorders and energy metabolism in rats with coronary heart disease
The effects of Buyang Huanwu Decoction on hemorheological disorders and energy metabolism in rats with coronary heart disease
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补阳还五汤对冠心病大鼠血液流变学紊乱及能量代谢的影响
DOI：
10.1016/j.jep.2011.05.008
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发表时间：
2011-09-01
影响因子：
5.4
通讯作者：
Ren, Feng
中科院分区：
文献类型：
--
作者：
Wang, Wei-Rong;Lin, Rong;Ren, Feng
民族药理学相关性：补阳还五汤（BYHWD）是一种中药方剂，已被公认为治疗冠心病气虚血瘀证的临床方药。白芍对气虚血瘀型冠心病血液流变学紊乱及能量代谢的影响尚不清楚。研究目的：探讨白芍对冠心病气虚血瘀证大鼠的改善作用是否与调节血液流变学紊乱和能量代谢有关。材料与方法：分别以25.68、12.84、6.42 g/kg BYHWD（混合生药g重量/kg体重）灌胃大鼠，每天1次，连续灌胃21 d。观察并记录体重、穷尽游泳时间和舌部特征。采用血液学分析仪测定全血粘度和血浆粘度。采用纤维蛋白原（Fbg）测定试剂盒测定血浆纤维蛋白原（Fbg）水平。采用半自动全血血小板分析仪检测腺苷二磷酸酶诱导的血小板聚集。采用LifeScan检测血糖（BC）水平。用分光光度计测定心脏组织Na+-K+- atp酶活性。结果：白芍汤改善了CUD大鼠气虚血瘀证的外在体征，包括体重、竭泳时间和舌质。25.68 g/kg BYHWD处理大鼠全血黏度以10 s（-1）的剪切速率降低（P < 0.05）， 25.68和12.84 g/kg BYHWD处理大鼠血浆黏度降低（P < 0.05）。25.68 g/kg BYHWD组大鼠血浆Fbg水平和血小板聚集降低（P < 0.01）。25.68和12.84 g/kg BYHWD组大鼠心脏BG水平降低，Na+-K+- atp酶活性升高（P < 0.01）。结论：白芍对气虚血瘀型冠心病大鼠的改善作用可能是通过改善血液流变学紊乱和能量代谢机制来实现的。2011爱思唯尔爱尔兰有限公司版权所有。
Ethnopharmacological relevance: Buyang Huanwu Decoction (BYHWD), a traditional Chinese medicine (TCM) formula, has been recognized as a clinical treatment for coronary heart disease (CHD) with qi deficiency and blood stasis syndrome. The effects of BYHWD on hemorheological disorders and energy metabolism in CHD with qi deficiency and blood stasis syndrome are still unclear.Aim of the study: To investigate whether the ameliorative effects of BYHWD on CHD rats with qi deficiency and blood stasis syndrome are associated with the regulation of hemorheological disorders and energy metabolism.Materials and methods: The rats were lavaged with 25.68, 12.84 and 6.42 g/kg BYHWD (g weight of mixed crude drugs/kg body weight), respectively, once a day for 21 days. The body weight, exhaustive swimming time and tongue characters were observed and recorded. The whole blood viscosity and plasma viscosity were determined by hematology analyzer. The level of fibrinogen (Fbg) in plasma was determined by using Fbg assay kit. The platelet aggregation induced by adenosine diphosphatase was measured by semiautomatic whole blood platelet analyzer. The level of blood glucose (BC) was determined by LifeScan. The activity of Na+-K+-ATPase in heart tissues was detected by spectrophotometer.Results: BYHWD improved the exterior signs of qi deficiency and blood stasis syndrome in rats with CUD, including the body weight, exhaustive swimming time and tongue quality. The whole blood viscosity in rats treated with 25.68 g/kg BYHWD decreased at the shear rate of 10 s(-1) (P < 0.05) and the plasma viscosity decreased in rats treated with 25.68 and 12.84 g/kg BYHWD (P < 0.05). The plasma Fbg level and the platelet aggregation decreased in rats treated with 25.68 g/kg BYHWD (P < 0.01). The results also revealed that the BG level decreased and the Na+-K+-ATPase activity in heart tissues increased in rats treated with 25.68 and 12.84 g/kg BYHWD (P < 0.01).Conclusion: The results suggest that the ameliorative effects of BYHWD on CHD rats with qi deficiency and blood stasis syndrome are mediated by the improvement of hemorheological disorders and energy metabolism. (C) 2011 Elsevier Ireland Ltd. All rights reserved.