NMDA and AMPA receptors on neocortical neurons are differentially distributed
NMDA and AMPA receptors on neocortical neurons are differentially distributed
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DOI：
10.1046/j.1460-9568.1998.00338.x
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发表时间：
1998-11-01
影响因子：
3.4
通讯作者：
Zieglgänsberger, W
中科院分区：
文献类型：
--
作者：
Dodt, HU;Frick, A;Zieglgänsberger, W
神经元表面谷氨酸受体亚型的分布与新皮质的突触激活和信号处理密切相关。作为一种新的方法，我们用红外视频显微镜结合光刺激或微离子电泳法在大鼠新皮质脑片上定位了N-甲基-D-天冬氨酸和alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic酸(AMPA)受体在V层锥体神经元上的分布。两种应用模式都显示了谷氨酸受体亚型的空间分布：神经元的胞体和近端的树突对NMDA高度敏感，而树突的更远端对AMPA更敏感。提示胞体附近的NMDA受体可能调节远端树突状细胞上AMPA受体激活所产生的突触信号的放大。
The distribution of glutamate receptor subtypes on the surface of neurons is highly relevant for synaptic activation and signal processing in the neocortex. As a novel approach we have used infra-red videomicroscopy in combination with photostimulation or microiontophoresis in brain slices of rat neocortex to map the distribution of N-methyl-D-aspartate (NMDA) and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors on pyramidal neurons of layer V. Both modes of application revealed a spatially distinct distribution of glutamate receptor subtypes: the soma and the proximal dendrite of neurons are highly sensitive to NMDA, whereas the more distal parts of the dendrite are more sensitive to AMPA. An implication is that NMDA receptors near the soma might regulate the amplification of synaptic signals resulting from AMPA receptor activation on remote dendritic sites.