Identification and quantification of (dihydro) hydroxycinnamic acids and their conjugates in potato by UHPLC-DAD-ESI-MSn
Identification and quantification of (dihydro) hydroxycinnamic acids and their conjugates in potato by UHPLC-DAD-ESI-MSn
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DOI：
10.1016/j.foodchem.2011.04.050
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发表时间：
2012-02-01
期刊：
影响因子：
8.8
通讯作者：
Gruppen, Harry
中科院分区：
文献类型：
--
作者：
Narvaez-Cuenca, Carlos-Eduardo;Vincken, Jean-Paul;Gruppen, Harry
通过UHPLC-DAD-ESI-MSn在马铃薯块茎提取物中鉴定和定量羟基肉桂酸缀合物（HCAcs）和二氢羟基肉桂酸缀合物（DHCAs）。通过筛选产物离子和中性损失并结合UV光谱进行HACs和DHACs鉴定。共检测到39种HCAcs/DHCAcs，其中包括17种以前在马铃薯中报道的HCAcs/DHCAcs。发现HCA是非共轭的，与含羟基化合物（包括己糖、奎尼酸和苹果酸）、含氨基化合物（如腐胺和章鱼胺）以及未知化合物相连。DHCA与精胺、亚精胺和尚未鉴定的化合物有关。绿原酸含量最高（25.43 +/- 0.49 mg/g DW），其次是隐绿原酸（7.31 +/- 0.38 mg/g DW），一种不可水解的芥子酸缀合物（2.80 +/- 0.06 mg/g DW）和新绿原酸（2.41 +/- 0.10 mg/g DW），总计占HCA/含DHCA化合物总浓度的83%（w/w）。碱性水解后释放的HCA的定量与未水解分子的定量很好地匹配。UHPLC-DAD-ESI-MSn方法显示马铃薯中HACs和DHACs的多样性比之前描述的更大。(C)2011爱思唯尔有限公司版权所有。
Hydroxycinnamic acid conjugates (HCAcs) and dihydrohydroxycinamic acid conjugates (DHCAcs) were identified and quantified in potato tuber extracts by UHPLC-DAD-ESI-MSn. The HCAcs and DHCAcs identification took place by screening for product ions and neutral losses in combination with UV spectra. Thirty-nine HCAcs/DHCAcs were detected, including 17 previously reported in potato. HCAs were found unconjugated, linked to hydroxyl-containing compounds including hexose, quinic acid and malic acid, to amino-containing compounds, such as putrescine and octopamine, and to unknown compounds. DHCAs were present linked to spermine, spermidine and to still unidentified compounds. Chlorogenic acid was the most abundant compound (25.43 +/- 0.49 mg/g DW) followed by cryptochlorogenic acid (7.31 +/- 0.38 mg/g DW), a non-hydrolyzable sinapic acid conjugate (2.80 +/- 0.06 mg/g DW) and neochlorogenic acid (2.41 +/- 0.10 mg/g DW), in total accounting for 83% (w/w) of the total concentration of HCAs/DHCAs-containing compounds. Quantifications of HCAs released after alkaline hydrolysis matched well with the quantification of the unhydrolyzed molecules. The UHPLC-DAD-ESI-MSn method showed a larger diversity of HCAcs and DHCAcs in potato than described before. (C) 2011 Elsevier Ltd. All rights reserved.