Lysophosphatidic acid inhibition of the accumulation of Pseudomonas aeruginosa PAO1 alginate, pyoverdin, elastase and LasA
Lysophosphatidic acid inhibition of the accumulation of Pseudomonas aeruginosa PAO1 alginate, pyoverdin, elastase and LasA
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DOI：
10.1099/00221287-148-6-1709
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发表时间：
2002-06-01
期刊：
影响因子：
2.8
通讯作者：
Cohen, PS
中科院分区：
文献类型：
--
作者：
Laux, DC;Corson, JM;Cohen, PS
铜绿假单胞菌的发病机制至少部分归因于其合成和分泌铁载体脓蛋白和两种锌金属蛋白酶、弹性蛋白酶和LasA的能力，以及其形成生物膜的能力，其中细菌细胞嵌入藻酸盐基质中。在本研究中，一种溶血磷脂，1-paimitoyl-2-羟基-sn-glycero-3-磷酸盐[也称为单棕榈酰磷脂酸（MPPA）]，在炎症渗出物中积累，被证明可以抑制铜绿假单胞菌PAO1海藻酸盐、弹性蛋白酶、LasA蛋白酶和铁载体脓毒蛋白的细胞外积累。 MPPA 还抑制生物膜的形成。 MPPA 的抑制作用独立于 rpoS 表达而发生，并且不影响自诱导剂 N-(3-氧代十二酰基) 高丝氨酸内酯和 N-丁酰-(L)-高丝氨酸内酯的积累，并且可能至少部分归因于 MPPA 结合二价阳离子的能力。
The pathogenesis of Pseudomonas aeruginosa is at least partially attributable to its ability to synthesize and secrete the siderophore pyoverdin and the two zinc metal loproteases elastase and LasA, and its ability to form biofilms in which bacterial cells are embedded in an alginate matrix. In the present study, a lysophospholipid, 1-paimitoyl-2-hydroxy-sn-glycero-3-phosphate [also called monopalmitoylphosphatidic acid (MPPA)], which accumulates in inflammatory exudates, was shown to inhibit the extracellular accumulation of P. aeruginosa PAO1 alginate, elastase, LasA protease and the siderophore pyoverdin. MPPA also inhibited biofilm formation. The inhibitory effects of MPPA occur independently of rpoS expression and without affecting the accumulation of the autoinducers N-(3-oxododecanoyl) homoserine lactone and N-butyryl-(L)-homoserine lactone, and may be due, at least in part, to the ability of MPPA to bind divalent cations.