CLONING AND CHARACTERIZATION OF 2 MEMBERS OF THE VERTEBRATE DLX GENE FAMILY
CLONING AND CHARACTERIZATION OF 2 MEMBERS OF THE VERTEBRATE DLX GENE FAMILY
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DOI：
10.1073/pnas.91.6.2250
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发表时间：
1994-03-15
影响因子：
11.1
通讯作者：
BONCINELLI, E
中科院分区：
文献类型：
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作者：
SIMEONE, A;ACAMPORA, D;BONCINELLI, E
已经在小鼠、青蛙和斑马鱼中克隆了许多Dir基因家族的脊椎动物基因。这些基因包含一个与Distalless基因相关的同源盒，该基因在果蝇胚胎发育中的头部和四肢中表达。我们克隆并研究了该家族的两个成员Dm和Dlx6在人和小鼠中的表达，人类的两个基因DLXS和DLX6在7号染色体7q22的一个区域以反向收敛的方式紧密连锁。类似地，人类的两个基因DLX1和DLX2在HOXD(以前的HOX4)基因座附近的2q32处紧密连锁，形成收敛构型。小鼠胚胎原位杂交实验显示，Dlx5和Dlx6在腹侧间脑和基底端脑的限制性区域表达，其分布与已报道的Dlx1和Dlx2的分布非常相似。DM和Dlx6的一个令人惊讶的特征是，在第一次软骨形成后，它们也在中期胚胎的所有骨骼结构中表达。这些基因的表达模式，以及它们的染色体定位，可能为研究合并有颅面和肢体缺陷的先天性疾病提供有用的线索。
A number of vertebrate genes of the Dir gene family have been cloned in mouse, frog, and zebrafish. These genes contain a homeobox related to that of Distalless, a gene expressed in the developing head and limbs of Drosophila embryos. We cloned and studied the expression of two members of this family, which we named DM and Dlx6, in human and mouse, The two human genes, DLXS and DLX6, are closely linked in an inverted convergent configuration in a region of chromosome 7, at 7q22. Similarly, the two human genes DLX1 and DLX2 are closely linked in a convergent configuration at 2q32, near the HOXD (previously HOX4) locus. In situ hybridization experiments in mouse embryos revealed expression of Dlx5 and Dlx6 mRNA in restricted regions of ventral diencephalon and basal telencephalon, with a distribution very similar to that reported for Dlx1 and Dlx2 mRNA. A surprising feature of DM and Dlx6 is that they are also expressed in all skeletal structures of midgestation embryos after the first cartilage formation. The expression pattern of these genes, together with their chromosome localization, may provide useful cues for the study of congenital disorders in which there is a combination of craniofacial and limb defects.