Detection and localization of periodontopathic bacteria in abdominal aortic aneurysms
Detection and localization of periodontopathic bacteria in abdominal aortic aneurysms
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DOI：
10.1016/j.ejvs.2004.08.010
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发表时间：
2004-11-01
影响因子：
5.7
通讯作者：
Ishikawa, I
中科院分区：
文献类型：
--
作者：
Kurihara, N;Inoue, Y;Ishikawa, I
目标.我们通过研究腹主动脉瘤（AAA）患者切除的腹主动脉瘤标本中牙周致病菌的存在，研究了牙周病和腹主动脉瘤之间可能的联系。前瞻性病例对照研究。材料和方法。32例AAA患者入组本研究。根据牙周袋探诊深度对牙周炎进行分类。应用聚合酶链反应（PCR）方法检测了32例主动脉瘤壁、16例附壁血栓、5例动脉粥样硬化闭塞和5例对照动脉组织中的7种牙周细菌。通过血栓动脉内膜切开术确定细菌在动脉粥样硬化壁中的定位。所有患者均患有牙周病，且大多数病例病情严重。PCR检测结果显示，86%的牙周组织标本细菌DNA阳性。对照标本中未检出细菌。结论动脉粥样硬化闭塞动脉内膜中层和外膜层均有细菌感染。牙周病细菌存在于AAA患者的患病动脉标本中的百分比很高，并且在整个动脉壁中发现。这些细菌可能在AAAs的发展中发挥作用和/或有助于削弱囊壁。
Objectives. We examined a possible link between periodontol disease and abdominal aortic aneurysm (AAA) by studying resected aneurysmal specimens from AAA patients for the presence of periodontitopathic bacteria.Design. Prospective case control study.Material and methods. Thirty-two AAA patients were enrolled in the study. Periodontitis was classified according to the probing depth of preriodontal pocket. Thirty-two aneurysmal walls, 16 mural thrombi, 5 atherosclerotic occlusive aorta and 5 control arterial tissue, were examined for 7 periodontal bacteria using polymerase chain reaction (PCR) method. The localization of the bacteria in the aneurysmall/atherosclerotic wall was determined by thromboendartercctomy.Results. All patients had periodontal disease, and most cases were severe. PCR examination of the aneurysmal specimens showed that 86% were positive for periodontal bacterial DNA. No bacteria were detected in the control specimens. The bacteria were found in both the intimall/medial layer and the adventitial layer of the aneurysmal wall but only in intimall/medial layer of the atherosclerotic occlusive aorta.Conclusion. Periodontopathic bacteria were present in a high percentage of specimens of diseased arteries from AAA patients and were found throughout the whole aneurysmal wall. These bacteria may play a role in the development of AAAs and/or contribute to weakening the aneurysmal wall.