Introduction of Cells into Porous Poly-l-Lactic Acid Scaffolds Using Impregnation Techniques
Introduction of Cells into Porous Poly-l-Lactic Acid Scaffolds Using Impregnation Techniques
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使用浸渍技术将细胞引入多孔聚 L-乳酸支架中
DOI：
10.1089/ten.tec.2020.0262
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Negishi Jun
中科院分区：
文献类型：
--
作者：
Yanagisawa Kotaro;Funamoto Seiichi;Hashimoto Yoshihide;Negishi Jun
含有细胞的多孔材料--通过在支架上种植细胞或凝胶化含有细胞的溶液而制备--已被广泛研究，以研究组织再生和三维培养。然而，这些方法不能将细胞引入吸水率低的多孔材料中，也不能将细胞引入需要细胞毒性溶剂或生产工艺的支架中。在本研究中，首先将三种不同的浸渍处理条件(真空、加压和真空压力浸渍：VPI)应用于细胞悬液，以评估每种处理对细胞的影响。三种浸渍处理后，成纤维细胞均附着在细胞培养皿上，并以与未处理细胞相同的方式增殖，证实三种浸渍处理不影响细胞功能。其次，将细胞导入低吸水率的聚L-乳酸支架中，采用相同的浸渍处理。经三种浸渍处理的聚乳酸支架的DNA含量明显高于浸渍处理，且DNA含量依次为真空处理、VPI处理和加压处理。此外，真空处理和VPI处理的聚乳酸支架中的DNA含量在培养的第1、3、5天都有所增加，证实了引入到聚乳酸支架中的细胞有增殖。这些结果表明，真空和VPI处理可能是将细胞引入多孔性材料的有用方法。
Porous materials containing cells—prepared via cell seeding on scaffolds or gelation of cell-containing solutions—have been widely studied to investigate tissue regeneration and three-dimensional cultures. However, these methods cannot introduce cells into porous materials that have low water absorption or scaffolds that require cytotoxic solvents or processes for their production. In this study, first, three different impregnation treatments conditions (vacuum, pressure, and vacuum pressure impregnation: VPI) were applied to cell suspensions to evaluate the effect of each treatment on cells. Following all three treatments, fibroblasts adhered to the cell culture dish and proliferated in the same manner as untreated cells, which confirmed that the three impregnation treatments did not affect cell function. Second, cells were introduced into a poly-l-lactic acid (PLA) scaffold, which has low water absorption, using the same impregnation treatments. The PLA scaffolds subjected to the three impregnation treatments that exhibited a significantly greater amount of DNA than those subjected to immersion treatments and showed increasing amounts of DNA in the order vacuum treatment > VPI treatment > pressure treatment. Furthermore, the amount of DNA in the vacuum-treated and VPI-treated PLA scaffolds increased on the first, third, and fifth days of culture, and it was confirmed that the cells introduced into the PLA scaffolds proliferated. These results suggest that vacuum and VPI treatments may be useful methods for introducing cells into porous materials.