Transient Gene Expression System Established in Porphyra yezoensis Is Widely Applicable in Bangiophycean Algae
Transient Gene Expression System Established in Porphyra yezoensis Is Widely Applicable in Bangiophycean Algae
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DOI：
10.1007/s10126-011-9367-6
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发表时间：
2011-10-01
影响因子：
3
通讯作者：
Mikami, Koji
中科院分区：
文献类型：
--
作者：
Hirata, Ryo;Takahashi, Megumu;Mikami, Koji
条斑紫菜瞬时基因表达系统的建立，为研究条斑紫菜的细胞过程奠定了基础。然而，目前还没有关于外源基因在其他红藻中成功表达的报道，这阻碍了对这些物种分子生物学的更广泛理解。因此，我们研究了条斑紫菜瞬时基因表达系统是否适用于其他红藻。结果表明，在条斑紫菜PyAct1启动子的控制下，密码子优化的GUS（命名为PyGUS）和植物适应的sGFP（S65 T）在6种紫菜的配子体细胞中成功表达，并且还在Bangia fuscopurpurea中表达，所有这些都被归类为Bangiophyceae。相比之下，在检查的Florideophycean藻类中没有表达细胞。结果表明，PyGUS和sGFP可作为报告基因，PyAct1基因5'上游可作为外源启动子在红海藻中瞬时表达，为外源基因在红海藻中的高效表达和转化提供了线索。
The establishment of transient gene expression systems in the marine red macroalga Porphyra yezoensis has been useful for the molecular analysis of cellular processes in this species. However, there has been no successful report about the expression of foreign genes in other red macroalgae, which has impeded the broader understanding of the molecular biology of these species. We therefore examined whether the P. yezoensis transient gene expression system was applicable to other red macroalgae. The results indicated that a codon-optimized GUS, designated PyGUS, and plant-adapted sGFP(S65T) were successfully expressed under the control of the P. yezoensis PyAct1 promoter in gametophytic cells of six Porphyra species and also in Bangia fuscopurpurea, all of which are classified as Bangiophyceae. In contrast, there were no reporter-expressing cells in the Florideophycean algae examined. These results indicate the availability of PyGUS and sGFP as reporters and the 5' upstream region of the PyAct1 gene as a heterologous promoter for transient gene expression in Bangiophycean algae, which could provide a clue to the efficient expression of foreign genes and transformation in marine red macroalgae.