A concentration gradient generator on a paper-based microfluidic chip coupled with cell culture microarray for high-throughput drug screening
A concentration gradient generator on a paper-based microfluidic chip coupled with cell culture microarray for high-throughput drug screening
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DOI：
10.1007/s10544-016-0054-2
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发表时间：
2016-02-01
影响因子：
2.8
通讯作者：
Kang, Yuejun
中科院分区：
文献类型：
--
作者：
Hong, Bo;Xue, Peng;Kang, Yuejun
受三维细胞培养纸平台的启发，设计并构建了一种含有药物浓度梯度的纸基微流控装置，用于高通量分析细胞对药物的反应。该药物梯度发生器用于产生阿霉素（DOX）作为模型药物的浓度梯度。将包封在胶原水凝胶中的HeLa细胞在装置储库中孵育，以基于DOX的空间受控释放来评估细胞活力。结果表明，通过纸纤维的药物扩散产生了药物浓度梯度，这影响了细胞活力。该药物筛选平台具有很大的机会，可应用于药物发现和诊断研究，在各种梯度浓度下同时和平行测试药物。
Inspired by the paper platforms for 3-D cell culture, a paper-based microfluidic device containing drug concentration gradient was designed and constructed for investigating cell response to drugs based on high throughput analysis. This drug gradient generator was applied to generate concentration gradients of doxorubicin (DOX) as the model drug. HeLa cells encapsulated in collagen hydrogel were incubated in the device reservoirs to evaluate the cell viability based on the controlled release of DOX spatially. It was demonstrated that drug diffusion through the paper fibers created a gradient of drug concentration, which influenced cell viability. This drug screening platform has a great opportunity to be applied for drug discovery and diagnostic studies with simultaneous and parallel tests of drugs under various gradient concentrations.