Alteration of T and null lymphocyte frequencies in the peripheral blood of human opiate addicts: in vivo evidence for opiate receptor sites on T lymphocytes.
Alteration of T and null lymphocyte frequencies in the peripheral blood of human opiate addicts: in vivo evidence for opiate receptor sites on T lymphocytes.
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人类阿片成瘾者外周血中 T 淋巴细胞和空淋巴细胞频率的改变：T 淋巴细胞上阿片受体位点的体内证据。
DOI：
10.4049/jimmunol.125.6.2539
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发表时间：
1980
影响因子：
4.4
通讯作者：
J. Mendelson
中科院分区：
文献类型：
--
作者：
R. McDonough;J. Madden;A. Falek;D. Shafer;M. Pline;D. Gordon;P. Bokos;J. Kuehnle;J. Mendelson
街头阿片类药物成瘾产生显着的抑郁症，在外周血中的总T淋巴细胞的绝对数量的淋巴细胞玫瑰花结绵羊红细胞（SRBC）的能力来衡量。与T细胞减少相关，无效淋巴细胞的绝对数量增加，但B淋巴细胞或总白色血细胞计数无显著变化。两个不同人群（n = 12和32）的T细胞值分别为31.8%和23.1%，而空细胞值分别为51.1%和57.6%。可比较对照群体（n = 18和10）的值为：T% = 70.7%和67.4%，无效% = 9.2%和14.5%。自我报告使用大麻不会显著改变细胞群的分布。用10（-6）至10（-7）M纳洛酮孵育成瘾衍生淋巴细胞1至3小时，通过使无效细胞表达SRBC受体，逆转T细胞抑制和无效细胞增加。环AMP和二丁酰环AMP也可以将无效细胞转化为T细胞。通过监测72小时培养物中PHA刺激生长的增加（通过氚化胸苷掺入DNA测定），还测量了空细胞向T淋巴细胞的转化。这些结果支持的假设，阿片类药物可以改变T淋巴细胞的数量和功能，在体内，这种改变可能会产生显着的变性街头阿片类药物成瘾者的免疫能力。
Street opiate addiction produces a significant depression in the absolute number of total T lymphocytes in peripheral blood as measured by the ability of the lymphocytes to rosette sheep red blood cells (SRBC). Associated with the decrease in T cells, there is an increase in the absolute number of null lymphocytes but no significant changes in B lymphocytes or total white blood cell count. The T cell values for 2 different populations of addicts (n = 12 and 32) are 31.8% and 23.1%, whereas the null cell values are 51.1% and 57.6%, respectively. The values for comparable control populations (n = 18 and 10) are: T% = 70.7% and 67.4%, and null % = 9.2% and 14.5%. Self-reported use of marihuana does not significantly alter the distribution of cell populations. A 1- to 3-hr incubation of addicted-derived lymphocytes with 10(-6) to 10(-7) M Naloxone reverses both T cell depression and null cell increase by allowing the null cells to express SRBC receptors. Cyclic AMP and dibutyryl cyclic AMP can also convert the null cells to T cells. The conversion of null to T lymphocytes has additionally been measured by monitoring the increase in PHA-stimulated growth in 72-hr cultures as determined by tritiated thymidine incorporation into DNA. These results support the hypothesis that opiates can alter T lymphocyte number and function in vivo, and that this alteration may produce a significant degeneration in the immune competence of street opiate addicts.