Field Hypothesis on the Self-regulation of Gene Expression
Field Hypothesis on the Self-regulation of Gene Expression
复制标题
基因表达自我调节的场假说
DOI：
10.1023/a:1021251125101
复制
发表时间：
2002
影响因子：
1.8
通讯作者：
K. Yoshikawa
中科院分区：
文献类型：
--
作者：
K. Yoshikawa
活细胞中基因表达的自我调节机制通常是通过考虑复杂的钥匙锁关系网络来解释的，事实上有大量的证据表明存在大量的钥匙锁关系。然而，在本文中我们强调，仅凭网络假说无法完全解释生活中的自我调节机制。最近，已经确定，大于几十个碱基对的单个巨型 DNA 分子在其高阶结构中经历了大的离散转变。很明显，与多种化学物质（例如多胺、小盐、ATP 和 RNA）的非特异性弱相互作用会导致 DNA 高阶结构的开/关切换。因此，除了钥匙和锁的网络之外，细胞环境的场参数应该在自我调节机制中发挥重要作用。这种由场参数诱导的构象转变可能与严格的开/关调节有关，而钥匙锁关系可能涉及更灵活的基因表达控制。
The mechanism of the self-regulation of gene expression in living cells is generally explained by considering complicated networks of key-lock relationships, and in fact there is a large body of evidence on a hugenumber of key-lock relationships. However, in the present article we stress that with the network hypothesis alone it is impossible to fully explain the mechanism of self-regulation in life. Recently, it has been established that individual giant DNA molecules, larger than several tens of kilo base pairs, undergo a large discrete transition in their higher-order structure. It has become clear that nonspecific weak interactions with various chemicals, suchas polyamines, small salts, ATP and RNA, cause on/off switching in the higher-order structure of DNA. Thus, the field parameters of the cellular environment should play important roles in the mechanism of self-regulation, in addition to networks of key and locks. This conformational transition induced by field parameters may be related to rigid on/off regulation, whereas key-lock relationships may be involved in a more flexible control of gene expression.