DsdA (D-serine deaminase): a new heterologous MX cassette for gene disruption and selection in Saccharomyces cerevisiae.
DsdA (D-serine deaminase): a new heterologous MX cassette for gene disruption and selection in Saccharomyces cerevisiae.
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DsdA（D-丝氨酸脱氨酶）：一种新的异源 MX 盒，用于酿酒酵母中的基因破坏和选择。
DOI：
10.1002/yea.1074
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
McCusker,JohnH
中科院分区：
文献类型：
--
作者：
Vorachek-Warren,MaraK;McCusker,JohnH
Dominant drug resistance markers offer experimental flexibility in the study ofSaccharomyces cerevisiaeby eliminating the dependence on auxotrophic mutations and, because they are phenotypically neutral, avoid the deleterious effects of auxotrophic mutations. We have developed a new dominant resistance marker,dsdAMX4, for use in the genetic manipulation ofS. cerevisiae. ThedsdAgene, which is derived fromEscherichia coliand encodes aD‐serine deaminase, confers toS. cerevisiaeresistance toD‐serine and the ability to useD‐serine as a nitrogen source. Here we describe the construction of adsdAMX4 cassette, capable of expression inS. cerevisiae, and the characterization of this new marker for use in chromosomal gene disruption. The unique selection properties of thedsdAMX4 cassette make it an important addition to the existing array ofS. cerevisiaegenetic tools. Copyright © 2004 John Wiley & Sons, Ltd.
影响因子：
5.8
作者：
OHASHI, M;FUJIO, N;MATSUO, H
通讯作者：
MATSUO, H