Biodegradable branched poly(ethylenimine sulfide) for gene delivery
Biodegradable branched poly(ethylenimine sulfide) for gene delivery
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DOI：
10.1016/j.biomaterials.2009.10.004
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发表时间：
2010-02-01
期刊：
影响因子：
14
通讯作者：
Park, Jong-Sang
中科院分区：
文献类型：
--
作者：
Koo, Heebeom;Jin, Geun-woo;Park, Jong-Sang
我们通过交联线性 PEIS 合成了可生物降解的 b-PEIS（支链聚（硫化乙烯亚胺））。我们通过调节交联剂双环氧化物的量来控制交联度和分子量。 b-PEIS 在还原条件（5 mm 谷胱甘肽溶液）下很容易降解，降解时间取决于交联程度。我们通过调节交联剂的量来控制 b-PEIS 的分子量，从而控制交联度。我们的滴定数据表明，双环氧化物交联之前或之后缓冲能力几乎没有损失。我们通过MALLS和凝胶电泳验证了该聚合物的降解情况，并根据细胞数据证实其具有高转染效率和低细胞毒性。通过图像恢复显微镜观察细胞内运输，证明 b-PEIS 不会在细胞内部积聚。 (C) 2009 Elsevier Ltd. 保留所有权利。
We synthesized biodegradable b-PEIS (branched poly(ethylenimine sulfide)) by crosslinking linear PEIS. We controlled the degree of crosslinking and molecular weight by adjusting the amount of the crosslinker, bisepoxide. The b-PEIS was readily degradable under reductive conditions (5 mm glutathione solution) and the degradation time was dependent on the degree of crosslinking. We controlled the molecular weights of the b-PEIS by regulating the amount of crosslinker and thus, the degree of crosslinking. Our titration data showed that there was almost no loss in buffering ability before or after bisepoxide crosslinking. We verified the degradation of this polymer by MALLS and gel electrophoresis, and confirmed that there was a high transfection efficiency and low cytotoxicity based on cellular data. Intracellular trafficking was observed by image restoration microscopy, demonstrating that b-PEIS does not accumulate in the cell interior. (C) 2009 Elsevier Ltd. All rights reserved.