A comparison of DigiGait™ and TreadScan™ imaging systems: assessment of pain using gait analysis in murine monoarthritis.
A comparison of DigiGait™ and TreadScan™ imaging systems: assessment of pain using gait analysis in murine monoarthritis.
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DOI：
10.2147/jpr.s52195
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发表时间：
2014
影响因子：
2.7
通讯作者：
Mahowald ML
中科院分区：
文献类型：
--
作者：
Dorman CW;Krug HE;Frizelle SP;Funkenbusch S;Mahowald ML
卡拉胶性关节炎是一种容易诱发的疼痛性急性关节炎模型，疼痛和炎症在3小时左右出现高峰。这种关节炎模型可以使用半定量诱发或非诱发疼痛评分系统进行评估。这些措施是主观的，往往是时间和劳动密集型的。利用快速评估工具对疼痛进行定量、非主观的评估是有益的。我们试图比较DigiGait™和TreadScan™系统，并验证两种步态分析平台通过步态行为的变化来检测卡拉胶诱导的单关节炎疼痛和镇痛反应。对无关节炎小鼠和卡拉胶诱导的关节炎小鼠进行了镇痛和不镇痛实验。将3%角叉菜胶注射到成年小鼠的膝关节，产生疼痛的膝关节。镇痛小鼠皮下注射0.015 mg/mL （0.5 mg/kg）丁丙诺啡。在DigiGait™或TreadScan™系统上捕获五秒钟的视频，并在计算步态参数后，使用学生非配对t检验进行比较。我们发现DigiGait™系统测量的步幅（挥杆时间、站立时间和步幅时间）明显长于TreadScan™。两个系统测量的可变性是相等的。两个系统的重现性不一致。虽然两种系统都检测到卡拉胶注射后步态测量的一些变化，但两种系统都没有发现任何变化。只有TreadScan™检测到镇痛后步态测量的正常化，但该系统无法检测到由于关节炎疼痛而改变的所有测量的正常化。花在分析上的时间取决于操作人员的经验。DigiGait™和TreadScan™系统都不能用于测量急性炎性单关节炎小鼠疼痛行为或镇痛反应的变化。
Carrageenan-induced arthritis is a painful acute arthritis model that is simple to induce, with peak pain and inflammation occurring at about 3 hours. This arthritis model can be evaluated using semiquantitative evoked or non-evoked pain scoring systems. These measures are subjective and are often time- and labor-intensive. It would be beneficial to utilize quantitative, nonsubjective evaluations of pain with rapid assessment tools. We sought to compare the DigiGait™ and TreadScan™ systems and to validate the two gait analysis platforms for detection of carrageenan-induced monoarthritis pain and analgesic response through changes in gait behavior. Non-arthritic mice and carrageenan-induced arthritic mice with and without analgesia were examined. A painful arthritic knee was produced by injection of 3% carrageenan into the knee joint of adult mice. Analgesic-treated mice were injected subcutaneously with 0.015 mg/mL (0.5 mg/kg) buprenorphine. Five-second videos were captured on the DigiGait™ or TreadScan™ system and, after calculating gait parameters, were compared using student’s unpaired t-test. We found the DigiGait™ system consistently measured significantly longer stride measures (swing time, stance time, and stride time) than did TreadScan™. Both systems’ measures of variability were equal. Reproducibility was inconsistent on both systems. While both systems detected alterations in some gait measures after carrageenan injection, none of the alterations were seen with both systems. Only the TreadScan™ detected normalization of gait measures after analgesia, but the system could not detect normalization across all measures that altered due to arthritis pain. Time spent on analysis was dependent on operator experience. Neither the DigiGait™ nor TreadScan™ system was useful for measuring changes in pain behaviors or analgesic responses in acute inflammatory monoarthritic mice.