Directional transfer of a multiple-allele male sterile line in Brassica campestris L. ssp. chinensis (L.) Makino var. rosularis Tsen et Lee.
Directional transfer of a multiple-allele male sterile line in Brassica campestris L. ssp. chinensis (L.) Makino var. rosularis Tsen et Lee.
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甘蓝型多等位基因雄性不育系的定向转移。 chinensis (L.) Makino var. 变种rosularis Tsen 等人。
DOI：
10.1270/jsbbs.64.149
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发表时间：
2014-06
期刊：
影响因子：
2.4
通讯作者：
Feng H
中科院分区：
文献类型：
--
作者：
Wang QS;Zhang X;Li CY;Liu ZY;Feng H
为生产五塔菜(Brassica campestris L.SSP.)杂交种。中国草(L.)Makino var.Rosularis Tsen et Lee)，设计了一种“定向转移程序”来选育五塔才多等位基因雄性不育系。奈白菜多等位基因雄性不育系以五大才(WT01)自交系为目标品系，以S01为雄性不育资源。采用轮回回交的方法同时传递雄性不育和其他植物学性状，并通过测交鉴定其基因型。雄性不育系由S01成功转入WT01。选育了植物学性状与WT01相似的新雄性不育系GMS-3。以GMS-3为母本配制了4个杂交组合。从新的雄性不育系中育成了一个包含最理想性状的杂交种(C1)。
To produce hybrid seeds of Wutacai (Brassica campestris L. ssp. chinensis (L.) Makino var. rosularis Tsen et Lee), a “directional transfer program” was designed to breed the multiple-allele male sterile line of Wutacai. A multiple-allele male sterile line of Naibaicai (Brassica campestris L. ssp. chinensis L., S01) was used as the male sterile resource, and an inbred line of Wutacai (WT01) was used as the target line. Recurrent backcrossing was employed to transfer the male sterility and other botanical traits simultaneously, while the genotype was identified through test crossing. The male sterility was successfully transferred from S01 to WT01. A new male sterile line, GMS-3, with similar botanical traits to WT01, was bred. Four hybrid combinations were generated with GMS-3 as the female parent. One hybrid (C1) that contained the most desirable traits was developed from the new male sterile line.