Mechanisms by which transcription can regulate somatic hypermutation.
Mechanisms by which transcription can regulate somatic hypermutation.
复制标题
转录调节体细胞超突变的机制。
DOI：
10.1038/sj.gene.6364053
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Minnick,MF
中科院分区：
文献类型：
--
作者：
Wright,BE;Schmidt,KH;Minnick,MF
Mechanisms for somatic hypermutation (SHM) have proven elusive. An actively transcribed substrate was analyzed to elucidate the role of stem–loop structures (SLSs) in SHM. Analysis with a new computer algorithm indicates that the location and mutability of a base are regulated by:(a) the extent to which it is unpaired,(b) the degree to which it is exposed by stabilization of SLSs containing and flanking it, and (c) the level of transcription that drives supercoiling, which creates and stabilizes SLSs containing unpaired bases vulnerable to mutation. New mechanisms are described by which transcription can differentially stabilize SLSs harboring targeted bases and establish specific base exposure patterns. Assuming that transcription levels correlate with the magnitude of superhelicity induced and the lengths of ssDNA forming SLSs, this analysis accounts for the location of all mutable bases during SHM.
