Three zebrafish MEF2 genes delineate somitic and cardiac muscle development in wild-type and mutant embryos
Three zebrafish MEF2 genes delineate somitic and cardiac muscle development in wild-type and mutant embryos
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DOI：
10.1016/0925-4773(96)00601-6
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发表时间：
1996-10
影响因子：
2.6
通讯作者：
B. Ticho;D. Stainier;M. Fishman;R. Breitbart
中科院分区：
文献类型：
--
作者：
B. Ticho;D. Stainier;M. Fishman;R. Breitbart
斑马鱼是脊椎动物胚胎学的重要实验系统，非常适合肌肉发育的分子生物学研究。转录因子，如MEF2，在发育过程中调节骨骼和心肌特异性基因。我们报告的三个斑马鱼MEF2基因的鉴定，像他们的哺乳动物同行，编码肌肉特异性启动子的DNA结合转录激活因子的功能。MEF2在斑马鱼中的表达模式定义了发育中体节和心脏中的离散细胞群，并且与其他物种相比，MEF2基因的发育调控具有机制意义。MEF2在两个突变体中表达的改变影响了体节的形成，这为了解胚胎中肌肉形成的控制提供了线索。
The zebrafish is an important experimental system for vertebrate embryology, and is well suited to the molecular analysis of muscle development. Transcription factors, such as the MEF2s, regulate skeletal and cardiac muscle-specific genes during development. We report the identification of three zebrafish MEF2 genes which, like their mammalian counterparts, encode factors that function as DNA-binding transcriptional activators of muscle specific promoters. The pattern of MEF2 expression in zebrafish defines discrete cell populations in the developing somites and heart and has mechanistic implications for developmental regulation of the MEF2 genes, when compared with other species. Alteration of MEF2 expression in two mutants affecting somitogenesis provides insight into the control of muscle formation in the embryo.