Molecular mapping of a new powdery mildew resistance gene Pm2b in Chinese breeding line KM2939
Molecular mapping of a new powdery mildew resistance gene Pm2b in Chinese breeding line KM2939
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中国育种系KM2939新白粉病抗性基因Pm2b的分子图谱
DOI：
10.1007/s00122-015-2457-5
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发表时间：
2015-04-01
影响因子：
5.4
通讯作者：
An, Diaoguo
中科院分区：
文献类型：
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作者：
Ma, Pengtao;Xu, Hongxing;An, Diaoguo
在冰草衍生系中鉴定了一个抗白粉病的Pm 2等位基因，并将其用于小麦育种。白粉病（Blumeria graminis f.小麦赤霉病（Bgt）是世界范围内最具破坏性的小麦病害之一。利用普通小麦及其近缘种的多种抗源进行抗病育种具有重要意义。KM 2939是一个苗期和成株期均表现高抗白粉病的新材料。它携带Pm2的单个显性白粉病抗性（Pm）等位基因，命名为Pm2b，先前的等位基因命名Pm2将被重新命名为Pm2a。Pm2b定位在染色体臂5DS上，两侧分别有序列特征扩增区（SCAR）标记SCAR112和SCAR203，遗传距离分别为0.5和1.3 cM。序列标记位点（STS）标记Mag6176和简单序列重复（SSR）标记Cfd81与SCAR203共分离。Pm2b在染色体臂5DS上的特异性不同于Pm2a、Pm46和PmLX 66的供体。等位性分析表明，Pm2b、Pm2a和PmLX 66是等位的。因此，Pm2b似乎是Pm2基因座上的一个新等位基因。利用这些紧密连锁的标记，可以加快Pm2b基因在小麦生产中的转移。
An allele ofPm2for wheat powdery mildew resistance was identified in a putativeAgropyron cristatum-derived line and used in wheat breeding programs. Powdery mildew (caused by Blumeria graminis f. sp. tritici, Bgt) is one of the most devastating wheat diseases worldwide. It is important to exploit varied sources of resistance from common wheat and its relatives in resistance breeding. KM2939, a Chinese breeding line, exhibits high resistance to powdery mildew at both the seedling and adult stages. It carries a single dominant powdery mildew resistance (Pm) allele of Pm2, designated Pm2b, the previous allelic designation Pm2 will be re-designated as Pm2a. Pm2b was mapped to chromosome arm 5DS and flanked by sequence characterized amplified region (SCAR) markers SCAR112 and SCAR203 with genetic distances of 0.5 and 1.3 cM, respectively. Sequence tagged site (STS) marker Mag6176 and simple sequence repeat (SSR) marker Cfd81 co-segregated with SCAR203. Pm2b differs in specificity from donors of Pm2a, Pm46 and PmLX66 on chromosome arm 5DS. Allelism tests indicated that Pm2b, Pm2a and PmLX66 are allelic. Therefore, Pm2b appears to be a new allele at the Pm2 locus. The closely linked markers were used to accelerate transfer of Pm2b to wheat cultivars in current production.