ION-MOLECULE REACTIONS FOR IMPROVED EFFECTIVE-MASS RESOLUTION IN ELECTROSPRAY MASS-SPECTROMETRY
ION-MOLECULE REACTIONS FOR IMPROVED EFFECTIVE-MASS RESOLUTION IN ELECTROSPRAY MASS-SPECTROMETRY
复制标题
DOI：
10.1021/ac00110a026
复制
发表时间：
1995-07-15
影响因子：
7.4
通讯作者：
GOERINGER, DE
中科院分区：
文献类型：
--
作者：
MCLUCKEY, SA;GOERINGER, DE
串联质谱被证明可以提高电喷雾质谱中的有效质量分辨率。该技术涉及在质荷比值的窄范围内选择离子群体，并允许离子进行质子转移反应。与通过质子转移形成的产物离子相关的质荷比的变化允许质量和电荷分配。该技术的成功依赖于在质荷比选择步骤中发生的特定电荷状态的离子的相对富集。该方法可用于扩展易于测量的聚合物质量范围，分析对于可靠的质量测量可能过于复杂的混合物，并且当阳离子的混合物存在于给定的电荷状态内时提高质量测量精度。该技术是用四极离子阱使用细胞色素c的多电荷离子，转移核糖核酸从E。大肠杆菌菌株W和合成的脱氧核糖核酸30聚体。
Tandem mass spectrometry is shown to improve the effective mass resolution in electrospray mass spectrometry. The technique involves selecting a population of ions within a narrow range of mass-to-charge values and allowing the ions to undergo proton transfer reactions. The shifts in mass-to-charge ratios associated with product ions formed by proton transfer allow for mass and charge assignment. The success of the technique relies on the relative enrichment of ions of a particular charge state that occurs in the mass-to-charge selection step. This approach can be used to extend the polymer mass range amenable to measurement, analyze mixtures that might otherwise be too complex for reliable mass measurements, and improve mass measurement precision when a mixture of cations is present within a given charge state. The technique is illustrated with a quadrupole ion trap using multiply-charged ions of cytochrome c, transfer ribonucleic acid from E. coli, strain W, and a synthetic deoxyribonucleic acid 30-mer.