High-performance tandem mass spectrometry: calibration and performance of linked scans of a four-sector instrument.
High-performance tandem mass spectrometry: calibration and performance of linked scans of a four-sector instrument.
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高性能串联质谱：四扇区仪器链接扫描的校准和性能。
DOI：
10.1021/ac00140a016
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发表时间：
1987
影响因子：
7.4
通讯作者：
Biemann,K
中科院分区：
文献类型：
--
作者：
Sato,K;Asada,T;Ishihara,M;Kunihiro,F;Kammei,Y;Kubota,E;Costello,CE;Martin,SA;Scoble,HA;Biemann,K
本文描述了四扇形串联质谱计的校准过程，其中第二质谱仪以恒定的82/E2比扫描。已经开发出硬件和软件来控制磁场，从而补偿磁体扫描中的任何不均匀性。制备了标准混合物，为正负模式的校准提供了参考质量。在任何一种模式下，方法都很简单，对于任何前体离子的质量高达3500只需要一条校准曲线。即使对于质量非常低的产品离子，透射率也达到最大，在整个范围内的质量分配精度优于0.3 u。用正离子模式下的肽和负离子模式下的糖脂来证明这种性能。测量由电子电离（El）产生的特定前体产生的产物离子的质量已经使用了许多年，主要用于研究质谱裂解过程或确认混合物中存在某种化合物。然而，各种“软”电离技术，如化学电离（Cl）、场电离或解吸（FI、FD），以及最近的快速原子轰击电离（FAB）或液体基质二次电离（liquid SIMS），所产生的光谱结构信息的缺乏，使得有必要从最初在软电离过程中产生的分子种类中产生具有结构意义的片段离子。这些碎片离子在所谓的亚稳态过程中自发形成（在分子离子产生后的几微秒内）
Procedures are described for the calibration of a four-sector tandem mass spectrometer for experiments In which the second mass analyzer Is scanned at constant 82/E2 ratio. Hardware and software have been developed to control the fields In such a manner that any nonuniformity In the magnet scan is compensated. Mixtures of standards have been pre-pared that provide reference masses for calibration in the positive and negative modes. In either mode, the method Is simple and only one calibration curve is required for any precursor ion up to mass 3500. Transmission Is maximized even for product Ions of very low mass and mass assignment accuracy of better than 0.3 u Is achieved over the entire range. The performance Is demonstrated with a peptide In the positive ion mode and a glycollpid In the negative mode.The measurement of the mass of product ions derived from a specific precursor generated by electron ionization (El) has been used for many years, chiefly to study mass spectrometric fragmentation processes or to confirm the presence of a certain compound in a mixture. However, the paucity of fragment ions and the corresponding lackof structural information of spectra produced by the various “soft” ionization techniques, such as chemical ionization (Cl), field ionization or desorption (FI, FD), and most recently, fast atom bombardment ionization (FAB) or secondary ionization with a liquid matrix (liquid SIMS) have made it necessary to generate structurally significant fragment ions from the molecular species produced originally in the soft ionization process. These fragment ions form either spontaneously (microseconds after the molecular ion has beengenerated) in the course of so-called metastable