LIPOSOMES COATED WITH CRYSTALLINE BACTERIAL-CELL SURFACE PROTEIN (S-LAYER) AS IMMOBILIZATION STRUCTURES FOR MACROMOLECULES
LIPOSOMES COATED WITH CRYSTALLINE BACTERIAL-CELL SURFACE PROTEIN (S-LAYER) AS IMMOBILIZATION STRUCTURES FOR MACROMOLECULES
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DOI：
10.1016/0005-2736(95)80013-6
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发表时间：
1995-05-04
影响因子：
3.4
通讯作者：
SLEYTR, UB
中科院分区：
文献类型：
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作者：
KUPCU, S;SARA, M;SLEYTR, UB
从凝结芽孢杆菌E38-66的结晶细胞表面层（S-层）分离的亚基在带正电荷的脂质体上重结晶。脂质体由二棕榈酰磷脂酰胆碱/胆固醇和硬脂胺组成。细菌表面上S层亚基的自然排列是斜（p2）晶格。亚基通过其内表面（其带有净负电荷）以与完整细胞上的晶格相同的方向附着于带正电荷的脂质体。一旦在脂质体上重结晶，S-层蛋白与戊二醛交联，随后用作大分子共价连接的基质。S层包覆的脂质体的高稳定性和将生物活性分子固定在晶体阵列上的可能性可以在各种不同的脂质体应用中提供潜力。
Isolated subunits from the crystalline cell surface layer (S-layer) of Bacillus coagulans E38-66 were recrystallized on positively charged liposomes. The liposomes were composed of dipalmitoylphosphatidylcholine/cholesterol and stearylamine. The natural arrangement of the S-layer subunits on the bacterial surface is as an oblique (p2) lattice. The subunits attached to positively charged liposomes by their inner face (which bears a net negative charge) in an orientation identical to the lattice on intact cells. The S-layer protein, once recrystallized on liposomes, was crosslinked with glutaraldehyde and subsequently used as a matrix for the covalent attachment of macromolecules. The high stability of S-layer-coated liposomes and the possibility for immobilizing biologically active molecules on the crystalline array may offer potential in various different liposome applications.