URINARY-EXCRETION OF ENDOTHELIN-1 IN NORMAL SUBJECTS AND PATIENTS WITH RENAL-DISEASE
URINARY-EXCRETION OF ENDOTHELIN-1 IN NORMAL SUBJECTS AND PATIENTS WITH RENAL-DISEASE
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DOI：
10.1038/ki.1991.38
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发表时间：
1991-02-01
影响因子：
19.6
通讯作者：
MARUMO, F
中科院分区：
文献类型：
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作者：
OHTA, K;HIRATA, Y;MARUMO, F
为了阐明尿内皮素-1 (ET-1) 的病理生理学意义，我们测量了 17 名肾病患者和 9 名正常受试者尿中 ET-1 样免疫反应性 (LI) 的排泄情况。 肾病患者 24 小时尿 ET-1-LI 排泄量（358 +/- 68 ng，平均值 +/- SE）显着（P < 0.005）高于正常受试者（77 +/- 5 ng）。 在肾病患者中，ET-1-LI 清除率 (C(ET)) 超过肌酐清除率 (C(CR))； C(ET)/C(CR) (305 +/- 81%) 显着 (P < 0.005) 高于正常受试者 (43 +/- 13%)。 肾病患者24小时尿中ET-1-LI的排泄量与N-乙酰基-β-D-氨基葡萄糖苷酶（r = 0.587，P < 0.05）、β-2-微球蛋白（r = 0.614，P < 0.01）和白蛋白（r = 0.484，P < 0.05）显着相关。 7名正常受试者静脉输注生理盐水（500ml）不影响尿ET-1排泄率。 这些数据表明，至少在肾病患者中，ET-1 的尿排泄主要来自肾小管分泌，并且肾小管部位的 ET-1 降解和/或重吸收也可能有助于肾脏对 ET-1 的处理。 因此，ET-1 的尿排泄应作为肾损伤的潜在标志物。
To elucidate the pathophysiological significance of urinary endothelin-1 (ET-1), we measured urinary excretion of ET-1-like immunoreactivity (LI) in 17 patients with renal disease and 9 normal subjects. Twenty-four hour urinary ET-1-LI excretion in patients with renal disease (358 +/- 68 ng, mean +/- SE) was significantly (P < 0.005) greater than that of normal subjects (77 +/- 5 ng). In patients with renal disease, ET-1-LI clearance (C(ET)) exceeded creatinine clearance (C(CR)); C(ET)/C(CR) (305 +/- 81%) was significantly (P < 0.005) greater than that of normal subjects (43 +/- 13%). The 24-hour urinary excretion of ET-1-LI in patients with renal disease showed significant correlation with that of N-acetyl-beta-D-glucosaminidase (r = 0.587, P < 0.05), beta-2-microglobulin (r = 0.614, P < 0.01) and albumin (r = 0.484, P < 0.05). Intravenous infusion of saline (500 ml) in seven normal subjects did not affect urinary ET-1 excretion rate. These data suggest that urinary excretion of ET-1 derives mainly from renal tubular secretion at least in patients with renal disease, and that degradation and/or reabsorption of ET-1 at the tubular site may also contribute to the renal handling of ET-1. Therefore, urinary excretion of ET-1 should serve as a potential marker for renal injury.