ANTIBODY AND CYTOTOXIC-T LYMPHOCYTE-RESPONSES OF HUMANS TO LIVE AND INACTIVATED INFLUENZA VACCINES
ANTIBODY AND CYTOTOXIC-T LYMPHOCYTE-RESPONSES OF HUMANS TO LIVE AND INACTIVATED INFLUENZA VACCINES
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DOI：
10.1099/0022-1317-58-2-273
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发表时间：
1982-01-01
影响因子：
3.8
通讯作者：
SCHILD, GC
中科院分区：
文献类型：
--
作者：
ENNIS, FA;QI, YH;SCHILD, GC
本文分析了36名志愿者对流感疫苗的抗体和hla限制性病毒特异性细胞毒性T淋巴细胞（CTL）反应。使用了三种疫苗：一种减毒活疫苗、两种灭活疫苗、一种全病毒疫苗和一种纯化表面抗原疫苗。采用斑块中和、单放射状溶血（SRH）和血凝抑制（HI）技术检测血凝素（HA）抗体。同时检测核蛋白抗体和基质抗原抗体。大多数志愿者对灭活疫苗中的HA有抗体反应，这是通过所有3种技术检测到的。在12名接种活病毒疫苗的患者中，9人在HI试验中没有检测到抗体反应，但在使用空斑中和试验时，其中4人有抗体反应。在检测低水平抗体时，SRH比HI更敏感，而斑块中和试验对检测低水平抗体最敏感。大多数志愿者的hla限制性流感特异性记忆CTL反应有所上升，但3名接受活疫苗的人没有任何技术的抗体反应，他们的细胞毒性T细胞活性也没有增加。3名志愿者，其中2名接种了活疫苗，CTL反应阳性，无抗体反应。
The antibody and HLA-restricted virus-specific cytotoxic T lymphocyte (CTL) responses to influenza vaccine of 36 volunteers were analyzed. Three vaccines were used: a live attenuated, and 2 types of inactivated, a whole virus and a purified surface antigen vaccine. Antibody to hemagglutinin (HA) was assayed using plaque neutralization, single-radial-hemolysis (SRH) and hemagglutination-inhibition (HI) techniques. Antibodies to nucleoprotein and matrix antigens were also measured. Most of the volunteers had antibody responses to the HA in the inactivated vaccines which were detected by all 3 techniques. Of the twelve recipients of the live virus vaccine, 9 did not have an antibody response detected by the HI test but 4 of these did have antibody responses when the plaque neutralization test was used. SRH was more sensitive than the HI for detecting low levels of antibody but the plaque neutralization test was the most sensitive for detecting low levels of antibody. Most volunteers had a rise in their HLA-restricted influenza-specific memory CTL response but 3 recipients of live vaccine who did not have an antibody response by any technique also did not have an increase in their cytotoxic T cell activity. Three volunteers, 2 of whom had received live vaccine, had a positive CTL response without antibody response.