Accumulation of eosinophils and T-lymphocytes in the lungs after exposure to pinewood dust
Accumulation of eosinophils and T-lymphocytes in the lungs after exposure to pinewood dust
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DOI：
10.1183/09031936.04.00059804
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发表时间：
2005-01-01
影响因子：
24.3
通讯作者：
Grunewald, J
中科院分区：
文献类型：
--
作者：
Gripenbäck, S;Lundgren, L;Grunewald, J
在木工行业接触木尘已被证明会导致各种呼吸系统疾病。为探讨松木粉尘对健康志愿者支气管肺泡灌洗液（BAL）和外周血细胞的影响，在全身暴露舱中对11名健康志愿者进行了1h的松木粉尘暴露。分别于染毒前26周和染毒后20 h对BALF液和血细胞进行差异计数，并检测肺泡巨噬细胞和T淋巴细胞活化、粘附和亚群标志物的表达。L-1（中位数（四分位距））至195.3（154.6-341.2）x 10（6）个细胞（.）L-1. BALF中T淋巴细胞浓度从3.8%（3.5-6.5%）增加到7.6%（4.9-11.2%），嗜酸性粒细胞浓度从0.0%（0.0-0.2%）增加到1.8%（0.6-3.5%）。肺部嗜酸性粒细胞、T淋巴细胞和肥大细胞（即对气道炎症至关重要的细胞）数量的增加可能与木工患呼吸系统疾病的风险增加有关。
Exposure to wood dust within the woodworking industry has been shown to cause a variety of respiratory disorders. The aim of this study was to investigate the cellular effects in bronchoalveolar lavage (BAL) fluid and peripheral blood from healthy individuals exposed to pinewood dust.Eleven healthy volunteers were exposed to pinewood dust for 1 h in a whole-body exposure chamber. BAL fluid and blood cells were differentially counted and the expression of activation, adhesion and subset markers on alveolar macrophages and T-lymphocytes was determined 26 weeks before and 20 h after the exposure.Following pinewood dust exposure, the total BAL fluid cell concentration increased from 81.4 (64.1-97.5) x 10(6) cells (.) L-1 (median (interquartile range)) to 195.3 (154.6-341.2) x 10(6) cells (.) L-1. The BAL fluid T-lymphocyte concentration increased from 3.8% (3.5-6.5%) to 7.6% (4.9-11.2%), and BAL fluid eosinophil concentration from 0.0% (0.0-0.2%) to 1.8% (0.6-3.5%).Inhalation of pinewood dust leads to the recruitment of inflammatory cells to the airways of healthy individuals. The increase in numbers of eosinophils, T-lymphocytes and mast cells, i.e. cells of crucial importance to airway inflammation, in the lungs may be related to the increased risk of developing respiratory disorders among woodworkers.