Quantitative proteomics identifies the core proteome of exosomes with syntenin-1 as the highest abundant protein and a putative universal biomarker.
Quantitative proteomics identifies the core proteome of exosomes with syntenin-1 as the highest abundant protein and a putative universal biomarker.
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定量蛋白质组学鉴定了外泌体的核心蛋白质组，其中syntenin-1是丰度最高的蛋白质，也是公认的通用生物标志物。
DOI：
10.1038/s41556-021-00693-y
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发表时间：
2021-06
影响因子：
21.3
通讯作者：
中科院分区：
文献类型：
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作者：
外泌体是来源于内体区室的细胞外囊泡，可能参与细胞间通讯。我们发现，外泌体的常用生物标志物是异质的，并且在不同的细胞类型中没有表现出普遍的效用。为了发现可以作为外泌体生物标志物的普遍存在和丰富的蛋白质，我们采用了基于Super-SILAC结合高分辨率质谱的无偏见和定量蛋白质组学方法。总之，无论细胞来源或分离方法如何，在外泌体的蛋白质组中始终定量了1，212种蛋白质。与细胞相比，一组22种蛋白质普遍富集，15种蛋白质在外泌体的蛋白质组中一致耗尽。在富集的蛋白质中，我们鉴定了生物发生相关的GTP酶和膜蛋白，如CD 47和ITGB 1。外泌体中耗尽的蛋白质组主要由核蛋白组成。我们将Syntenin-1鉴定为来自不同细胞来源的外泌体中最高一致丰度的蛋白质，其也存在于不同物种和生物流体的外泌体中，具有作为外泌体的推定通用生物标志物候选物的潜在效用。我们的研究提供了一个全面的核心蛋白质的定量图谱无处不在的外来体，可以作为科学界致力于研究外来体的资源。
Exosomes are extracellular vesicles derived from the endosomal compartment with potential involvement in intercellular communication. We found that frequently used biomarkers of exosomes are heterogeneous and do not exhibit universal utility across different cell types. To uncover ubiquitous and abundant proteins that can serve as biomarkers of exosomes, we employed an unbiased and quantitative proteomic approach based on Super-SILAC coupled to high-resolution mass spectrometry. In total, 1,212 proteins were consistently quantified in the proteome of exosomes irrespective of the cellular source or isolation method. A cohort of 22 proteins were universally enriched, and 15 proteins consistently depleted in the proteome of exosomes when compared to cells. Among the enriched proteins, we identified biogenesis-related, GTPases, and membrane proteins, such as CD47 and ITGB1. The cohort of depleted proteins in exosomes was predominantly composed of nuclear proteins. We identified Syntenin-1 as the highest consistently abundant protein in exosomes from distinct cellular origins, which was also present in exosomes across different species and biofluids, with potential utility as a putative universal biomarker candidate for exosomes. Our study provides a comprehensive quantitative atlas of core proteins ubiquitous to exosomes that can serve as a resource for the scientific community dedicated to the study of exosomes.
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DOI：
10.1126/science.aau6977
发表时间：
2020-02-07
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Kalluri R;LeBleu VS
通讯作者：
LeBleu VS
影响因子：
3.7
作者：
Altadill, Tatiana;Campoy, Irene;Cheema, Amrita K.
通讯作者：
Cheema, Amrita K.
影响因子：
13.6
作者：
Gonzales, Patricia A.;Pisitkun, Trairak;Knepper, Mark A.
通讯作者：
Knepper, Mark A.
影响因子：
11.2
作者：
Hwang, Rosa F.;Moore, Todd;Logsdon, Craig D.
通讯作者：
Logsdon, Craig D.
影响因子：
4.4
作者：
Cox, Juergen;Neuhauser, Nadin;Mann, Matthias
通讯作者：
Mann, Matthias