Possible extension of the activity spectrum of Bacillus thuringiensis strains through chemical additives
Possible extension of the activity spectrum of Bacillus thuringiensis strains through chemical additives
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DOI：
10.1111/j.1439-0418.1986.tb00862.x
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发表时间：
1986-01
影响因子：
1.9
通讯作者：
H. Salama;M. Foda;A. Sharaby
中科院分区：
文献类型：
--
作者：
H. Salama;M. Foda;A. Sharaby
已经开发了一些化学方法来扩展苏云金芽孢杆菌变种δ-内毒素的活性谱。kurstaki HD-1;从而包括Spodoptera littoralis（Boisd.）通常被认为是对这种品种的弱易感性。这些程序是基于掺入一些选定的基本无毒的化合物，这些化合物与内毒素具有不同的作用模式，导致其增强作用。采用人工饲料和天然寄主植物进行试验。在所测试的化合物中，某些无机盐、氨基酸和酰胺、芳香族化合物、蛋白质增溶剂、硼酸钠和单宁酸显示出对B的内毒素活性的显著增强。苏云kurstaki HD-1;与S.海滨鱼这些发现证实了检测内毒素活性谱的可能扩展，并可用于填补目前不同B谱存在的空白。苏云金杆菌菌株的寄主范围和生物学特性。根据所得结果讨论了这些化合物的作用方式，并结合其应用特点进行了分析。
Some chemical procedures have been developed to extend the activity spectrum of δ‐endotoxin of Bacillus thuringiensis var. kurstaki HD‐1; so as to include Spodoptera littoralis (Boisd.) normally known as being weakly susceptible to this variety. These procedures were based on the incorporation of some selected essentially non toxic compounds with different modes of action with the endotoxin resulting in its potentiation. Experiments were made using artificial diet and natural host plants. Among the compounds tested, some inorganic salts, amino acids and amides, aromatic compounds, protein solubilizing agents, sodium borate and tannic acid showed a remarkable potentiation in the endotoxin activity of B. thuringiensis var. kurstaki HD‐1; vs. S. littoralis. These findings confirm the possible extension of the activity spectrum of the tested endotoxin and may be used to fill the gaps now existing with respect to the spectra of different B. thuringiensis strains, their host ranges and biological specificities. The mode of action of these compounds has been discussed in the light of the results obtained and the results are analysed in correlation with their application feasibilities.