Effect of plasma proteins on buprenorphine transfer across dually perfused placental lobule.
Effect of plasma proteins on buprenorphine transfer across dually perfused placental lobule.
复制标题
血浆蛋白对丁丙诺啡跨双灌注胎盘小叶转运的影响。
DOI：
10.1080/14767050802610328
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Ahmed,MahmoudS
中科院分区：
文献类型：
--
作者：
Nanovskaya,TatianaN;Bowen,RobinS;Patrikeeva,SvetlanaL;Hankins,GaryDV;Ahmed,MahmoudS
目的：本研究的目的是确定人血清白蛋白（HSA）和α-酸性糖蛋白（AAG）对丁丙诺啡（BUP）经胎盘转运和分布的影响。BUP与标记化合物安替比林（AP）共灌注。在每个实验中，添加放射性标记的同位素[3 H]-BUP和[14 C]-AP以提高其检测限。人血浆蛋白，HSA和AAG，分别加入到母体和胎儿的电路，并在其生理浓度在母体和胎儿的循环接近term.Results。BUP的经胎盘转移，在血浆蛋白的情况下，是一个两步的过程：第一个是它被合体滋养层从母体回路摄取，第二个是它从组织转移/释放到胎儿回路。在灌注介质中加入HSA仅影响BUP转移的第二步，但AAG影响两个步骤。HSA和AAG的联合作用是没有不同的观察到在存在后者单独的。Conclusions. BUP结合到循环AAG可能有一个重要的作用，在药物从母体到胎儿循环的转移。
Objective.The aim of this investigation is to determine the effect of human serum albumin (HSA) and α-acid glycoprotein (AAG) on buprenorphine (BUP) transplacental transfer and distribution.Methods.The technique of dual perfusion of placental lobule (DPPL) was utilised. BUP was co-perfused with the marker compound antipyrine (AP). In each experiment, the radiolabelled isotopes [3H]-BUP and [14C]-AP were added to enhance their detection limits. Human plasma proteins, HSA and AAG, were added to both the maternal and fetal circuits separately and in combination at their physiological concentrations in maternal and fetal circulations close to term.Results.Transplacental transfer of BUP, in absence of plasma proteins, is a two-step process: the first is its uptake by the syncytiotrophoblast from the maternal circuit, and the second is its transfer/release from the tissue to the fetal circuit. The addition of HSA to the perfusion medium affected only the second step of BUP transfer, but AAG affected both steps. The combined effect of HSA and AAG was not different from that observed in presence of the latter alone.Conclusions.Binding of BUP to circulating AAG could have an important role in the transfer of the drug from the maternal to fetal circulation.