Effect of 1α,25(OH)2D3-Treated M1 and M2 Macrophages on Cell Proliferation and Migration Ability in Ovarian Cancer
Effect of 1α,25(OH)2D3-Treated M1 and M2 Macrophages on Cell Proliferation and Migration Ability in Ovarian Cancer
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1α,25(OH)2D3 处理的 M1 和 M2 巨噬细胞对卵巢癌细胞增殖和迁移能力的影响
DOI：
10.1080/01635581.2021.2014903
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发表时间：
2021-12-06
影响因子：
2.9
通讯作者：
Li, Bingyan
中科院分区：
文献类型：
--
作者：
Guo, Yi;Jiang, Fei;Li, Bingyan
维生素D3，1α，25-脱氧乙他的生物活性形式D3 [1α，25（OH）（2）D-3]，发挥了多效效应，包括骨矿化，抗肿瘤，抗肿瘤，以及免疫调节剂。这项研究旨在探讨1个α，25（oh）（2）D-3对肿瘤相关巨噬细胞（TAM）浸润对卵巢癌的潜在影响。首先，在存在凤梨12-溶质酸13-乙酸酯（PMA）的情况下，人类单核细胞THP-1细胞分化为巨噬细胞（M0）。在体内，1个α，25（OH）（2）D-3不仅逆转了M2巨噬细胞的极化，而且还降低了由M2巨噬细胞诱导的卵巢癌细胞的增殖和迁移能力。此外，1α，25（OH）（2）D-3显着降低了M2巨噬细胞中TGF-beta 1和MMP-9的分泌。但是，在1个α，25（OH）（2）D-3处理过的M1巨噬细胞中未观察到显着影响。在体内，维生素D3具有1个α，25（OH）（2）D-3处理的M2巨噬细胞对肿瘤发生的抑制作用。此外，我们通过荟萃分析进行了TAM与卵巢癌患者预后不良的关联，这表明M2巨噬细胞比例较高与卵巢癌的预后较差有关。总的来说，这些结果确定了1个α，25（OH）（2）D-3处理的M1和M2巨噬细胞在卵巢癌中细胞增殖和迁移能力上的不同作用。
The biological active form of vitamin D3, 1 alpha,25-dehydroxyvitamin D3 [1 alpha,25(OH)(2)D-3], exerts pleiotropic effects including bone mineralization, anti-tumor, as well as immunomodulator. This study aimed to explore the potential impact of 1 alpha,25(OH)(2)D-3 on tumor-associated macrophages (TAMs) infiltration in ovarian cancer. Firstly, human monocytic THP-1 cells were differentiated into macrophages (M0) in the presence of phorbol 12-myristate 13-acetate (PMA). In Vivo, 1 alpha,25(OH)(2)D-3 not only reversed the polarization of M2 macrophages, but also decreased the proliferation and migration abilities of ovarian cancer cells induced by M2 macrophages supernatant. Furthermore, 1 alpha,25(OH)(2)D-3 dramatically decreased the secretion of TGF-beta 1 and MMP-9 in M2 macrophages. However, no significant effect was observed in 1 alpha,25(OH)(2)D-3 treated M1 macrophages. In Vivo, vitamin D3 had an inhibitive effect of 1 alpha,25(OH)(2)D-3-treated M2 macrophages on tumorigenesis. In addition, we conducted the association of TAMs with the poor prognosis of patients with ovarian cancer by meta-analysis, which suggested the higher proportion of M2 macrophages was related to the poorer prognosis in ovarian cancer. Collectively, these results identified distinct roles of 1 alpha,25(OH)(2)D-3 treated M1 and M2 macrophages on cell proliferation and migration abilities in ovarian cancer.