Immunolocalization of water channel aquaporins in the nasal olfactory mucosa
Immunolocalization of water channel aquaporins in the nasal olfactory mucosa
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DOI：
10.1679/aohc.69.1
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发表时间：
2006-03-01
影响因子：
--
通讯作者：
Takata, K
中科院分区：
文献类型：
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作者：
Ablimit, A;Matsuzaki, T;Takata, K
水通道蛋白（Aquaporins，AQP）是一种在多种组织和器官中表达的膜水通道蛋白，其作用是将水和小分子溶质跨膜转运。我们提出了抗体的水通道蛋白亚型特异性合成肽，并调查其在大鼠鼻嗅觉和呼吸道粘膜的表达。采用免疫组化和免疫印迹法检测AQP1、AQP3、AQP4和AQP5。AQP1主要表达于嗅、呼吸道粘膜血管内皮细胞及周围结缔组织细胞。AQP 1可能参与水穿过血管壁的转移。在嗅上皮中，嗅感觉细胞中未检测到AQP。相反，AQP3丰富的嗅觉上皮，在那里它被定位在支持细胞和基底细胞。AQP3的表达主要局限于呼吸道上皮的基底细胞。与此相反，AQP4丰富的呼吸上皮，但其丰度仅限于基底细胞的嗅上皮。在Bowman腺中，AQP5定位于分泌腺泡细胞的顶膜，而AQP3和AQP4则定位于基底外侧膜。导管细胞也有类似的定位。这些结果表明水通道蛋白在嗅上皮中具有不同的定位模式。支持细胞和基底细胞中的AQP3和AQP4可能在嗅觉感觉细胞周围特定微环境的产生和维持中起重要作用。Bowman腺中的AQP3、AQP4和AQP5可能在分泌物中起作用，以在嗅树突的顶面产生用于气味接收的微环境。
Aquaporins (AQPs), membrane water channel proteins expressed in various tissues and organs, serve in the transfer of water and small solutes across the membrane. We raised antibodies to AQPs using isoform-specific synthetic peptides and surveyed their expression in the rat nasal olfactory and respiratory mucosae. AQP1, AQP3, AQP4, and AQP5 were detected by immunohistochemical and immunoblotting analyses. AQP1 was expressed in the endothelial cells of blood vessels and the surrounding connective tissue cells in the olfactory and respiratory mucosae. AQP1 may be involved in water transfer across the blood vessel wall. In the olfactory epithelium, no AQP was detected in the olfactory sensory cells. Instead, AQP3 was abundant in the olfactory epithelium, where it was localized in the supporting cells and basal cells. Expression of AQP3 was mostly restricted to the basal cells in the respiratory epithelium. In marked contrast, AQP4 was abundant in the respiratory epithelium, but its abundance was limited to the basal cells in the olfactory epithelium. In the Bowman's gland, AQP5 was localized in the apical membrane in the secretory acinar cells, whereas AQP3 and AQP4 were found in the basolateral membrane. Similar localization was seen in its duct cells. These results showed a distinct localization pattern for AQPs in the olfactory epithelium. AQP3 and AQP4 in the supporting cells and basal cells may play an important role in generating and maintaining the specific microenvironment around the olfactory sensory cells. AQP3, AQP4, and AQP5 in the Bowman's gland may serve in the secretion to generate the microenvironment at the apical surface of the olfactory dendrites for odorant reception.