THE CISA CISTRON OF BACILLUS-SUBTILIS SPORULATION GENE SPOIVC ENCODES A PROTEIN HOMOLOGOUS TO A SITE-SPECIFIC RECOMBINASE
THE CISA CISTRON OF BACILLUS-SUBTILIS SPORULATION GENE SPOIVC ENCODES A PROTEIN HOMOLOGOUS TO A SITE-SPECIFIC RECOMBINASE
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DOI：
10.1128/jb.172.2.1092-1098.1990
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发表时间：
1990-02-01
影响因子：
3.2
通讯作者：
KOBAYASHI, Y
中科院分区：
文献类型：
--
作者：
SATO, T;SAMORI, Y;KOBAYASHI, Y
测定了枯草芽孢杆菌产孢基因spoIVC cisA的核苷酸序列，发现其编码的蛋白质由500个氨基酸残基组成，计算分子量为57，481，与十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定的基因产物大小一致。该蛋白的N-末端区域的氨基酸序列与位点特异性DNA重组酶同源。将带有spoIVC cisA基因的3，6-内切酶EcoRI片段与从各阶段细胞中制备的EcoRI限制性染色体DNA杂交表明，DNA重排仅发生在孢子形成开始后3小时与spoIVC cisA相邻区域的母细胞中。这一结果与Stragier等人（P. Stragier，B.孔克尔湖Kroos和R. Losick，Science 243：507-512，1989）。DNA重排的时间与spoIVC cisA基因表达的时间非常一致。在spoIVC cisA突变体中未观察到DNA重排。这些结果强烈表明，spoIVC顺式A基因编码的位点特异性DNA重组酶具有非常重要的作用，在孢子形成。
The nucleotide sequence of the sporulation gene spoIVC cisA in Bacillus subtilis was determined and found to encode a protein of 500 amino acid residues with a calculated molecular weight of 57,481, which is in good agreement with the size of the gene product estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The amino acid sequence of the N-terminal region of this protein is homologous to the site-specific DNA recombinases. Hybridization of a 3,6-kilobase EcoRI fragment carrying the spoIVC cisA gene with the EcoRI-restricted chromosomal DNA prepared from cells of various stages showed that DNA rearrangement occurs only in the mother cell in the region adjacent to spoIVC cisA 3 h after the initiation of sporulation. This result coincides with that of Stragier et al. (P. Stragier, B. Kunkel, L. Kroos, and R. Losick, Science 243:507-512, 1989). The timing of the DNA rearrangement coincides very well with the timing of spoIVC cisAgene expression. The DNA rearrangement was not observed in spoIVC cisA mutants. These results strongly suggest that the spoIVC cisA gene encodes a site-specific DNA recombinase having a very important role in sporulation.