Immunoglobulin switch circular DNA in the mouse infected with Nippostrongylus brasiliensis: evidence for successive class switching from mu to epsilon via gamma 1.
Immunoglobulin switch circular DNA in the mouse infected with Nippostrongylus brasiliensis: evidence for successive class switching from mu to epsilon via gamma 1.
复制标题
感染巴西圆线虫的小鼠中的免疫球蛋白转换环状 DNA：通过 γ 1 从 mu 到 epsilon 连续类别转换的证据。
DOI：
10.1073/pnas.87.20.7829
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发表时间：
1990
影响因子：
11.1
通讯作者：
Sakano,H
中科院分区：
文献类型：
--
作者：
Yoshida,K;Matsuoka,M;Usuda,S;Mori,A;Ishizaka,K;Sakano,H
我们的特点是免疫球蛋白开关环状DNA感染的线虫寄生虫巴西日本圆线虫的小鼠。在淋巴结中鉴定出两种环状DNA，它们是免疫球蛋白重链恒定区（CH）基因开关重组的切除产物。一种是C mu和C gamma 1之间的重组体（gamma 1圆圈），另一种是C gamma 1和C gamma 1之间的重组体（gamma 1圆圈）。在S环中，在S环和S γ 1序列之间插入一小段开关μ（S μ）序列。插入的S μ序列可能是之前从C μ到C γ 1的转换的痕迹。这些发现表明，寄生虫感染可以诱导类转换重组在一个连续的方式，首先从C μ到C γ 1，然后从C γ 1到C γ。
We have characterized immunoglobulin switch circular DNA in mice infected with the nematode parasite Nippostrongylus brasiliensis. Two kinds of circular DNA were identified in the lymph nodes as excision products of switch recombination of immunoglobulin heavy-chain constant region (CH) genes. One is a recombinant between C mu and C gamma 1 (gamma 1 circle), and the other is a recombinant between C gamma 1 and C epsilon (epsilon circle). In the epsilon circle, a short piece of switch mu (S mu) sequence was inserted between S epsilon and S gamma 1 sequences. The inserted S mu sequence could be a trace of the preceding switch from C mu to C gamma 1. These findings indicate that parasitic infection can induce class switch recombinations in a successive manner, first from C mu to C gamma 1, and then from C gamma 1 to C epsilon.