Social dominance-related major urinary proteins and the regulatory mechanism in mice
Social dominance-related major urinary proteins and the regulatory mechanism in mice
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小鼠社会优势相关主要尿蛋白及其调控机制
DOI：
10.1111/1749-4877.12165
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发表时间：
2015-11-01
影响因子：
3.3
通讯作者：
Zhang, Jianxu
中科院分区：
文献类型：
--
作者：
Guo, Huifen;Fang, Qi;Zhang, Jianxu
主要尿蛋白（MUP）已被证明是小鼠体内的非挥发性雄性信息素。在这里，我们的目的是阐明 MUP 与优势层次之间的关系以及潜在的分子机制。支配-服从关系是通过长期的二元相遇而建立的。我们发现，在尿蛋白水平和肝脏 mRNA 水平上，与从属雄性小鼠相比，主要 MUP（包括 Mup20）的表达在雄性小鼠中增强，表明 MUP 可能预示着雄性小鼠的社会地位。同时，从属雄性小鼠中肝脏促肾上腺皮质激素释放激素受体2（CRHR2）的mRNA水平高于显性雄性小鼠。去势也增强了 CRHR2 的表达，但抑制了 MUP 的表达。 CRHR2激动剂治疗降低了肝脏中MUP的表达。然而，男性社会地位未能对血清睾酮和皮质酮及其受体的mRNA表达产生显着影响。这些发现表明，一些 MUP，尤其是 Mup20，可能构成潜在的优势信息素，并可能被肝 CRHR2 下调，而肝 CRHR2 可能独立于雄激素或皮质酮系统。
Major urinary proteins (MUPs) have been proven to be non-volatile male pheromones in mice. Here, we aimed to elucidate the relationship between MUPs and dominance hierarchy, and the underlying molecular mechanisms. Dominance-submission relationship was established by chronic dyadic encountering. We found that at the urinary protein level and hepatic mRNA level, the expression of major MUPs, including Mup20, was enhanced in dominant males compared with subordinate males, indicating that MUPs might signal the social status of male mice. Meanwhile, the mRNA level of hepatic corticotropin releasing hormone receptor 2 (CRHR2) was higher in subordinate male mice than in dominant male mice. Castration also enhanced the expression of CRHR2, but suppressed that of MUPs. CRHR2 agonist treatment reduced the expression of MUPs in liver. However, male social status failed to exert significant influence on serum testosterone and corticosterone as well as the mRNA expression of their receptors. These findings reveal that some MUPs, especially Mup20, might constitute potential dominance pheromones and could be downregulated by hepatic CRHR2, which is possibly independent of androgen or corticosterone systems.