Pulsed DC Electric Field-Induced Differentiation of Cortical Neural Precursor Cells
Pulsed DC Electric Field-Induced Differentiation of Cortical Neural Precursor Cells
复制标题
DOI：
10.1371/journal.pone.0158133
复制
发表时间：
2016-06-28
期刊：
影响因子：
3.7
通讯作者：
Cheng, Ji-Yen
中科院分区：
文献类型：
--
作者：
Chang, Hui-Fang;Lee, Ying-Shan;Cheng, Ji-Yen
我们报告了仅由直流（DC）脉冲刺激诱导的神经干细胞和祖细胞的分化。成年哺乳动物大脑中的神经干细胞和祖细胞是开发治疗性神经再生策略的有希望的候选细胞。神经干细胞和祖细胞的分化取决于体内各种环境因素，如神经生长因子和内源性EF。在这项研究中，我们证明小鼠神经干细胞和祖细胞 (mNPC) 的形态和表型变化可以仅通过暴露于方波直流脉冲（幅度为 300 mV/mm，频率为 100 Hz）来诱导。直流脉冲刺激48小时，连续监测mNPCs的形态变化。直流脉冲刺激48小时后，初级突起的长度和分支数量显着增加。经过直流脉冲处理后，mNPCs 在干细胞维持培养基中同时分化为神经元、星形胶质细胞和少突胶质细胞。我们的结果表明，简单的直流脉冲治疗可以控制 NPC 的命运。通过进一步的研究，直流脉冲可用于操纵 NPC 分化，并可用于开发利用 NPC 治疗神经系统疾病的治疗策略。
We report the differentiation of neural stem and progenitor cells solely induced by direct current (DC) pulses stimulation. Neural stem and progenitor cells in the adult mammalian brain are promising candidates for the development of therapeutic neuroregeneration strategies. The differentiation of neural stem and progenitor cells depends on various in vivo environmental factors, such as nerve growth factor and endogenous EF. In this study, we demonstrated that the morphologic and phenotypic changes of mouse neural stem and progenitor cells (mNPCs) could be induced solely by exposure to square-wave DC pulses (magnitude 300 mV/mm at frequency of 100-Hz). The DC pulse stimulation was conducted for 48 h, and the morphologic changes of mNPCs were monitored continuously. The length of primary processes and the amount of branching significantly increased after stimulation by DC pulses for 48 h. After DC pulse treatment, the mNPCs differentiated into neurons, astrocytes, and oligodendrocytes simultaneously in stem cell maintenance medium. Our results suggest that simple DC pulse treatment could control the fate of NPCs. With further studies, DC pulses may be applied to manipulate NPC differentiation and may be used for the development of therapeutic strategies that employ NPCs to treat nervous system disorders.