Reconstitution of surfactant activity by using the 6 kDa apoprotein associated with pulmonary surfactant.
Reconstitution of surfactant activity by using the 6 kDa apoprotein associated with pulmonary surfactant.
复制标题
使用与肺表面活性剂相关的 6 kDa 脱辅基蛋白重建表面活性剂活性。
DOI：
10.1042/bj2360085
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
F. Possmayer
中科院分区：
文献类型：
--
作者：
S. H. Yu;F. Possmayer
牛肺表面活性物质的脂质提取物含有6 kDa的载脂蛋白，但缺乏35 kDa的载脂蛋白，可以模仿肺表面活性物质的脉动气泡表面活性仪的基本特征。重构的表面活性剂可以通过将含有6kDa脱辅基蛋白和磷脂酰甘油的油酸级分与磷脂酰胆碱组合来产生。用蛋白水解酶处理含蛋白质的部分会破坏其功效。这些结果表明，6 kDa载脂蛋白的存在可以解释一些基本的物理和生物学特性的肺表面活性物质。免疫扩散研究表明，相反，早期的建议，6 kDa的载脂蛋白是不相关的主要表面活性剂载脂蛋白，具有35 kDa的分子量的结构。
Lipid extracts of bovine pulmonary surfactant containing the 6 kDa apoprotein, but lacking the 35 kDa apoprotein, can mimic the essential characteristics of pulmonary surfactant on a pulsating-bubble surfactometer. Reconstituted surfactant can be produced by combining silicic acid fractions containing 6 kDa apoprotein and phosphatidylglycerol with phosphatidylcholine. Treatment of the protein-containing fraction with proteolytic enzymes abolishes its efficacy. These results indicate that the presence of the 6 kDa apoprotein can account for some of the essential physical and biological characteristics of pulmonary surfactant. Immunodiffusion studies indicate that, contrary to earlier suggestions, the 6 kDa apoprotein is not structurally related to the major surfactant apoprotein that has a molecular mass of 35 kDa.