Two distinct human POM121 genes: Requirement for the formation of nuclear pore complexes
Two distinct human POM121 genes: Requirement for the formation of nuclear pore complexes
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DOI：
10.1016/j.febslet.2007.09.021
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发表时间：
2007-10-16
期刊：
影响因子：
3.5
通讯作者：
Imamoto, Naoko
中科院分区：
文献类型：
--
作者：
Funakoshi, Tomoko;Maeshima, Kazuhiro;Imamoto, Naoko
Pom 121是脊椎动物细胞核孔复合体（NPC）的膜组成成分之一。与携带单个POM 121基因的啮齿动物细胞不同，人类细胞在染色体7q11.23上具有多个POM 121基因位点，这是人类进化过程中该区域复杂片段复制的结果。在HeLa细胞中，两个“全长”Pom 121由两个不同的遗传基因座转录和翻译。RNAi实验表明，两种Pom 121蛋白的有效去除显著减少了核膜上组装的NPC。Pom 121缺失也诱导NPC聚集，表明其在NPC结构/组织维持中的作用。(c)2007年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
Pom121 is one of the integral membrane components of the nuclear pore complex (NPC) in vertebrate cells. Unlike ro- dent cells carrying a single POM121 gene, human cells possess multiple POM121 gene loci on chromosome 7q11.23, as a consequence of complex segmental-duplications in this region during human evolution. In HeLa cells, two "full-length" Pom121 are transcribed and translated by two distinct genetic loci. RNAi experiments showed that efficient depletion of both Pom121 proteins significantly reduces assembled NPCs on nuclear envelope. Pom121-depletion also induced clustering of NPCs, indicating its role on maintenance of NPC structure/organization. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.