Control mechanism of the circadian clock for timing of cell division in vivo
Control mechanism of the circadian clock for timing of cell division in vivo
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DOI：
10.1126/science.1086271
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发表时间：
2003-10-10
期刊：
影响因子：
56.9
通讯作者：
Okamura, H
中科院分区：
文献类型：
--
作者：
Matsuo, T;Yamaguchi, S;Okamura, H
许多哺乳动物组织中的细胞分裂与一天中的特定时间有关，但生物钟如何控制这种时间尚不清楚。在这里，我们显示在再生肝脏（小鼠）的生物钟控制细胞周期相关基因的表达，反过来调节活性细胞周期蛋白B1-Cdc 2激酶的表达，有丝分裂的关键调节因子。在这些基因中，wee 1的表达直接受生物钟的分子组成部分的调控。相比之下，在单细胞中，生物钟的振荡与细胞周期无关。因此，细胞内的昼夜节律时钟可以直接和单向地控制增殖细胞中的细胞分裂周期。
Cell division in many mammalian tissues is associated with specific times of day, but just how the circadian clock controls this timing has not been clear. Here, we show in the regenerating liver ( of mice) that the circadian clock controls the expression of cell cycle-related genes that in turn modulate the expression of active Cyclin B1-Cdc2 kinase, a key regulator of mitosis. Among these genes, expression of wee1 was directly regulated by the molecular components of the circadian clockwork. In contrast, the circadian clockwork oscillated independently of the cell cycle in single cells. Thus, the intracellular circadian clockwork can control the cell-division cycle directly and unidirectionally in proliferating cells.